Overview

  • Product nameAnti-GCKR antibody - N-terminal
    See all GCKR primary antibodies
  • Description
    Rabbit polyclonal to GCKR - N-terminal
  • Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human GCKR (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).

  • Positive control
    • A375 cell lysate and human hepatocarcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab71627 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).
IHC-P 1/50 - 1/100.

Target

  • FunctionInhibits glucokinase by forming an inactive complex with this enzyme.
  • Tissue specificityFound in liver and pancreas. Not detected in muscle, brain, heart, thymus, intestine, uterus, adipose tissue, kidney, adrenal, lung or spleen.
  • Sequence similaritiesBelongs to the GCKR family.
    Contains 2 SIS domains.
  • Information by UniProt
  • Database links
  • Alternative names
    • FGQTL5 antibody
    • GCK antibody
    • GCKR antibody
    • GCKR_HUMAN antibody
    • GKRP antibody
    • GLRE antibody
    • glucokinase (hexokinase 4) regulator antibody
    • Glucokinase antibody
    • Glucokinase regulator antibody
    • Glucokinase regulatory protein antibody
    • Hexokinase type IV antibody
    • Hexokinase-4 antibody
    • Hexokinase-D antibody
    • HK IV antibody
    • HK4 antibody
    • OTTHUMP00000158533 antibody
    see all

Anti-GCKR antibody - N-terminal images

  • Anti-GCKR antibody - N-terminal (ab71627) at 1/100 dilution + A375 cell lysate at 12.5 µg

    Predicted band size : 69 kDa
    Observed band size : 69,70 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 33 kDa,40 kDa. We are unsure as to the identity of these extra bands.
  • ab71627 at 1/50 dilution, staining GCKR in human hepatocarcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and DAB staining.

References for Anti-GCKR antibody - N-terminal (ab71627)

ab71627 has not yet been referenced specifically in any publications.

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