Overview

  • Product nameAnti-GCLC antibody
    See all GCLC primary antibodies
  • Description
    Rabbit polyclonal to GCLC
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human GCLC.

    (The immunizing peptide is available as ab41462)

  • Positive control
    • This antibody gave a positive signal in the following Human Tissue Lysates: Liver, Kidney and Lung (Adenocarcinoma). This antibody gave a positive result in IHC in the following FFPE tissue: Human lung adenocarcinoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab41463 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa).

Target

Anti-GCLC antibody images

  • All lanes : Anti-GCLC antibody (ab41463) at 1 µg/ml

    Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 2 : Human lung tissue lysate (adenocarcinoma) - insoluble fraction (female, 80 years) (ab42923)
    Lane 3 : Human kidney tissue lysate - total protein (ab30203)
    Lane 4 : Human liver tissue lysate - total protein (ab29889)

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 73 kDa
    Observed band size : 73 kDa
    Additional bands at : 58 kDa,60 kDa. We are unsure as to the identity of these extra bands.

References for Anti-GCLC antibody (ab41463)

This product has been referenced in:
  • Bailey-Downs LC  et al. Liver-specific knockdown of IGF-1 decreases vascular oxidative stress resistance by impairing the Nrf2-dependent antioxidant response: a novel model of vascular aging. J Gerontol A Biol Sci Med Sci 67:313-29 (2012). WB ; Mouse . Read more (PubMed: 22021391) »
  • Videla LA  et al. Thyroid hormone-induced cytosol-to-nuclear translocation of rat liver Nrf2 is dependent on Kupffer cell functioning. ScientificWorldJournal 2012:301494 (2012). WB ; Rat . Read more (PubMed: 22649286) »

See all 6 Publications for this product

Product Wall

Thank you for contacting us.
Unfortunately, the antibody was discontinued by the supplying lab.
The immunogens are derived from very different positions (ab17926 from AA295-313 of rat, ab41463 within residues 50 - 150 of Human GCLC). However,...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"