Overview

  • Product name
  • Description
    Rabbit polyclonal to GCLC
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human GCLC.

    (The immunizing peptide is available as ab41462)

  • Positive control
    • This antibody gave a positive signal in the following Human Tissue Lysates: Liver, Kidney and Lung (Adenocarcinoma). This antibody gave a positive result in IHC in the following FFPE tissue: Human lung adenocarcinoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab41463 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa).

Target

Images

  • All lanes : Anti-GCLC antibody (ab41463) at 1 µg/ml

    Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 2 : Human lung tissue lysate (adenocarcinoma) - insoluble fraction (female, 80 years) (ab42923)
    Lane 3 : Human kidney tissue lysate - total protein (ab30203)
    Lane 4 : Human liver tissue lysate - total protein (ab29889)

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 73 kDa
    Observed band size : 73 kDa
    Additional bands at : 58 kDa,60 kDa. We are unsure as to the identity of these extra bands.

References

This product has been referenced in:
  • Shanmugam G  et al. Constitutive activation of Nrf2 induces a stable reductive state in the mouse myocardium. Redox Biol 12:937-945 (2017). WB ; Mouse . Read more (PubMed: 28482326) »
  • Kumar RR  et al. Abrogation of Nrf2 impairs antioxidant signaling and promotes atrial hypertrophy in response to high-intensity exercise stress. J Transl Med 14:86 (2016). IHC-Fr ; Mouse . Read more (PubMed: 27048381) »

See all 14 Publications for this product

Customer reviews and Q&As

Thank you for contacting us.
Unfortunately, the antibody was discontinued by the supplying lab.
The immunogens are derived from very different positions (ab17926 from AA295-313 of rat, ab41463 within residues 50 - 150 of Human GCLC). However,...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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