Overview

  • Product name
  • Description
    Rabbit polyclonal to GCLC
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide corresponding to internal region of rat GCLC protein

  • Positive control
    • Kidney and Testis

Properties

Applications

Our Abpromise guarantee covers the use of ab53179 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use at an assay dependent concentration.

Target

Images

  • ab53179 staining mouse embryo cells by ICC/IF.  Cells were PFA fixed and permeabilized in Triton-X prior to blocking with 5% serum for 1 hr at 25°C.  The primary antibody was diluted 1/500 in PBS with 0.5% goat serum and incubated with the sample for 18 hrs at 4°C.  A FITC-conjugated goat anti-rabbit was used as the secondary antibody.  Note spindle staining in cells undergoing mitosis

    See Abreview

  • Anti-GCLC antibody (ab53179) at 1/25 dilution + lysate prepared from kidney

References

This product has been referenced in:
  • Chaiprasongsuk A  et al. Activation of Nrf2 Reduces UVA-Mediated MMP-1 Upregulation via MAPK/AP-1 Signaling Cascades: The Photoprotective Effects of Sulforaphane and Hispidulin. J Pharmacol Exp Ther 360:388-398 (2017). Read more (PubMed: 28011874) »
  • Chaisiriwong L  et al. A Case-Control Study of Involvement of Oxidative DNA Damage and Alteration of Antioxidant Defense System in Patients with Basal Cell Carcinoma: Modulation by Tumor Removal. Oxid Med Cell Longev 2016:5934024 (2016). IHC . Read more (PubMed: 27057281) »

See all 7 Publications for this product

Customer reviews and Q&As

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab41463. Currently ab41463 is backordered. You should expect to recei...

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Thank you for submitting your completed complaint questionnaire. It does appear that this antibody is not detecting GCLC as expected. If you have purchased this antibody within the last 6 months or so I would be happy to offer you a free replacement, c...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Liver)
Loading amount
20 µg
Specification
Liver
Treatment
Acetaminophen for 24 hrs
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Aug 23 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Embryo)
Specification
Embryo
Fixative
Paraformaldehyde
Permeabilization
Yes - Triton-X
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Feb 11 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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