Overview

  • Product nameAnti-GCLC antibody
    See all GCLC primary antibodies
  • Description
    Rabbit polyclonal to GCLC
  • Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide corresponding to internal region of rat GCLC protein

  • Positive control
    • Kidney and Testis

Properties

Applications

Our Abpromise guarantee covers the use of ab53179 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use at an assay dependent concentration.

Target

Anti-GCLC antibody images

  • ab53179 staining mouse embryo cells by ICC/IF.  Cells were PFA fixed and permeabilized in Triton-X prior to blocking with 5% serum for 1 hr at 25°C.  The primary antibody was diluted 1/500 in PBS with 0.5% goat serum and incubated with the sample for 18 hrs at 4°C.  A FITC-conjugated goat anti-rabbit was used as the secondary antibody.  Note spindle staining in cells undergoing mitosis

    See Abreview

  • Anti-GCLC antibody (ab53179) at 1/25 dilution + lysate prepared from kidney

References for Anti-GCLC antibody (ab53179)

This product has been referenced in:
  • Jain SK  et al. Hydrogen sulfide upregulates glutamate-cysteine ligase catalytic subunit, glutamate-cysteine ligase modifier subunit, and glutathione and inhibits interleukin-1ß secretion in monocytes exposed to high glucose levels. Metab Syndr Relat Disord 12:299-302 (2014). WB ; Human . Read more (PubMed: 24665821) »
  • Jain SK  et al. Vitamin D and L-cysteine levels correlate positively with GSH and negatively with insulin resistance levels in the blood of type 2 diabetic patients. Eur J Clin Nutr N/A:N/A (2014). Read more (PubMed: 24961547) »

See all 3 Publications for this product

Product Wall

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab41463. Currently ab41463 is backordered. You should expect to recei...

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Thank you for submitting your completed complaint questionnaire. It does appear that this antibody is not detecting GCLC as expected. If you have purchased this antibody within the last 6 months or so I would be happy to offer you a free replacement, c...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Liver)
Loading amount 20 µg
Specification Liver
Treatment Acetaminophen for 24 hrs
Gel Running Conditions Non-reduced Non-Denaturing (Native)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Aug 23 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Embryo)
Specification Embryo
Fixative Paraformaldehyde
Permeabilization Yes - Triton-X
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Feb 11 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"