Overview

  • Product nameAnti-GCLC antibody
    See all GCLC primary antibodies
  • Description
    Mouse monoclonal to GCLC
  • Tested applicationsSuitable for: WB, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 528-638 of Human GCLC.

Properties

Applications

Our Abpromise guarantee covers the use of ab55435 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 73 kDa.
ICC/IF Use a concentration of 10 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • PathwaySulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2.
  • Involvement in diseaseDefects in GCLC are the cause of hemolytic anemia (HAGGSD) [MIM:230450].
  • Sequence similaritiesBelongs to the glutamate--cysteine ligase type 3 family.
  • Information by UniProt
  • Database links
  • Alternative names
    • EC 6.3.2.2 antibody
    • Gamma ECS antibody
    • Gamma glutamylcysteine synthetase antibody
    • Gamma-ECS antibody
    • Gamma-glutamylcysteine synthetase antibody
    • GCL antibody
    • Gclc antibody
    • GCS antibody
    • GCS heavy chain antibody
    • GLCL antibody
    • GLCLC antibody
    • Glutamate cysteine ligase catalytic subunit antibody
    • Glutamate--cysteine ligase catalytic subunit antibody
    • GSH1_HUMAN antibody
    see all

Anti-GCLC antibody images



  • Predicted band size : 73 kDa
  • ab55435 at 10 ug/ml staining GCLC in human Hella cells by Immunocytochemistry/ Immunofluorescence.

  • Overlay histogram showing Jurkat cells stained with ab55435 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55435, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-GCLC antibody (ab55435)

This product has been referenced in:
  • Gu F  et al. Impaired synthesis and antioxidant defense of glutathione in the cerebellum of autistic subjects: alterations in the activities and protein expression of glutathione-related enzymes. Free Radic Biol Med 65:488-96 (2013). Read more (PubMed: 23892356) »
  • Agyeman AS  et al. Transcriptomic and proteomic profiling of KEAP1 disrupted and sulforaphane-treated human breast epithelial cells reveals common expression profiles. Breast Cancer Res Treat 132:175-87 (2012). WB ; Rat . Read more (PubMed: 21597922) »

See all 3 Publications for this product

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The optimal dilution for antibodies are dependent on many factors and that is the reason why we provide a range of suggest dilutions.
The factors include: amount of total protein per lane expression level of the sp...

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