The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. Ready-to-use for 30 min at RT
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM
citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
Use at an assay dependent concentration.
PathwaySulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2.
Involvement in diseaseDefects in GCLC are the cause of hemolytic anemia (HAGGSD) [MIM:230450].
Sequence similaritiesBelongs to the glutamate--cysteine ligase type 3 family.