Overview

  • Product nameAnti-GCLM antibody
    See all GCLM primary antibodies
  • Description
    Mouse monoclonal to GCLM
  • Tested applicationsSuitable for: WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-275 of Human GCLM (Gamma Glutamylcysteine Synthetase regulatory subunit).

Applications

Our Abpromise guarantee covers the use of ab55436 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • Tissue specificityIn all tissues examined. Highest levels in skeletal muscle.
  • PathwaySulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2.
  • Sequence similaritiesBelongs to the aldo/keto reductase family. Glutamate--cysteine ligase light chain subfamily.
  • Information by UniProt
  • Database links
  • Alternative names
    • Gamma ECS regulatory subunit antibody
    • Gamma-ECS regulatory subunit antibody
    • Gamma-glutamylcysteine synthetase regulatory subunit antibody
    • GCLM antibody
    • GCS light chain antibody
    • GLCLR antibody
    • Glutamate cysteine ligase regulatory subunit antibody
    • Glutamate--cysteine ligase modifier subunit antibody
    • Glutamate--cysteine ligase regulatory subunit antibody
    • GSC light chain antibody
    • GSH0_HUMAN antibody
    see all

Anti-GCLM antibody images

  • GCLM (ab55436) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • Overlay histogram showing HeLa cells stained with ab55436 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55436, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

References for Anti-GCLM antibody (ab55436)

This product has been referenced in:
  • Ishikado A  et al. 4-Hydroxy hexenal derived from docosahexaenoic acid protects endothelial cells via Nrf2 activation. PLoS One 8:e69415 (2013). WB ; Mouse . Read more (PubMed: 23936010) »
  • Jackson RM & Gupta C Hypoxia and kinase activity regulate lung epithelial cell glutathione. Exp Lung Res 36:45-56 (2010). Read more (PubMed: 20128681) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"