• Product name
  • Description
    Mouse monoclonal to GCLM
  • Tested applications
    Suitable for: WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-275 of Human GCLM (Gamma Glutamylcysteine Synthetase regulatory subunit).

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.


Our Abpromise guarantee covers the use of ab55436 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.


  • Tissue specificity
    In all tissues examined. Highest levels in skeletal muscle.
  • Pathway
    Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2.
  • Sequence similarities
    Belongs to the aldo/keto reductase family. Glutamate--cysteine ligase light chain subfamily.
  • Information by UniProt
  • Database links
  • Alternative names
    • Gamma ECS regulatory subunit antibody
    • Gamma-ECS regulatory subunit antibody
    • Gamma-glutamylcysteine synthetase regulatory subunit antibody
    • GCLM antibody
    • GCS light chain antibody
    • GLCLR antibody
    • Glutamate cysteine ligase regulatory subunit antibody
    • Glutamate--cysteine ligase modifier subunit antibody
    • Glutamate--cysteine ligase regulatory subunit antibody
    • GSC light chain antibody
    • GSH0_HUMAN antibody
    see all


  • GCLM (ab55436) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • Overlay histogram showing HeLa cells stained with ab55436 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55436, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.


This product has been referenced in:
  • Singh A  et al. Small Molecule Inhibitor of NRF2 Selectively Intervenes Therapeutic Resistance in KEAP1-Deficient NSCLC Tumors. ACS Chem Biol 11:3214-3225 (2016). WB ; Human . Read more (PubMed: 27552339) »
  • Sha LK  et al. Loss of Nrf2 in bone marrow-derived macrophages impairs antigen-driven CD8(+) T cell function by limiting GSH and Cys availability. Free Radic Biol Med 83:77-88 (2015). Mouse . Read more (PubMed: 25687825) »

See all 5 Publications for this product

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