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Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer Preservative: None
Constituents: 2% Sucrose, PBS
Concentration information loading...
Purity Immunogen affinity purified
Immunizing Peptide (Blocking)
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Use a concentration of 1 µg/ml. Predicted molecular weight: 31 kDa.Can be blocked with
Human GCLM peptide (ab111661). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Tissue specificity In all tissues examined. Highest levels in skeletal muscle.
Pathway Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2.
Sequence similarities Belongs to the aldo/keto reductase family. Glutamate--cysteine ligase light chain subfamily.
Information by UniProt
Gamma ECS regulatory subunit antibody
Gamma-ECS regulatory subunit antibody
Gamma-glutamylcysteine synthetase regulatory subunit antibody
Anti-GCLM antibody images
Western blot - Anti-GCLM antibody (ab81445)
Predicted band size : 31 kDa Western blot analysis of 40µg Mouse heart lysate (lanes 1 and 2) labeling GCLM with ab81445 at 1:1000. A HRP-conjugated anti-rabbit (1:10000) was used as the secondary antibody.
Western blot - GCLM antibody (ab81445)
Anti-GCLM antibody (ab81445) at 1 µg/ml (in 5% skim milk / PBS buffer) + MCF7 Cell Lysate at 10 µg Secondary HRP conjugated anti-Rabbit IgG at 1/50000 dilution Predicted band size : 31 kDa Observed band size : 31 kDa Gel Concentration: 12%
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-GCLM antibody(ab81445)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling GCLM with ab81445 at 2.5 µg/ml.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"