Overview

  • Product name
  • Description
    Rabbit polyclonal to GCLM
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    A synthetic peptide corresponding to a region within internal amino acids 180-229 QVKPNSNQVN LASCCVMPPD LTAFAKQFDI QLLTHNDPKE LLSEASFQEA of Human GCLM.

  • Positive control
    • MCF7 Cell Lysate

Applications

Our Abpromise guarantee covers the use of ab81445 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 31 kDa.Can be blocked with Human GCLM peptide (ab111661). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Anti-GCLM antibody images



  • Predicted band size : 31 kDa
    Western blot analysis of 40µg Mouse heart lysate (lanes 1 and 2) labeling GCLM with ab81445 at 1:1000. A HRP-conjugated anti-rabbit (1:10000) was used as the secondary antibody.
  • Anti-GCLM antibody (ab81445) at 1 µg/ml (in 5% skim milk / PBS buffer) + MCF7 Cell Lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 31 kDa
    Observed band size : 31 kDa
    Gel Concentration: 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling GCLM with ab81445 at 2.5 µg/ml.

References for Anti-GCLM antibody (ab81445)

This product has been referenced in:
  • Shanmugam G  et al. Constitutive activation of Nrf2 induces a stable reductive state in the mouse myocardium. Redox Biol 12:937-945 (2017). WB ; Mouse . Read more (PubMed: 28482326) »
  • Shanmugam G  et al. A biphasic effect of TNF-a in regulation of the Keap1/Nrf2 pathway in cardiomyocytes. Redox Biol 9:77-89 (2016). Read more (PubMed: 27423013) »

See all 2 Publications for this product

Product Wall

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Heart (young & old mouse))
Loading amount
30 µg
Specification
Heart (young & old mouse)
Gel Running Conditions
Non-reduced Denaturing (10-12%)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
Username

Mr. Mtuhtu S.Gounder

Verified customer

Submitted Nov 15 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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