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Rabbit polyclonal to GCLM
HumanPredicted to work with:
Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
A synthetic peptide corresponding to a region within internal amino acids 180-229 QVKPNSNQVN LASCCVMPPD LTAFAKQFDI QLLTHNDPKE LLSEASFQEA of Human GCLM.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
Concentration information loading...
Immunogen affinity purified
Immunizing Peptide (Blocking)
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use at an assay dependent concentration.
||Use a concentration of 1 µg/ml. Predicted molecular weight: 31 kDa.Can be blocked with Human GCLM peptide (ab111661). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
In all tissues examined. Highest levels in skeletal muscle.
Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2.
Belongs to the aldo/keto reductase family. Glutamate--cysteine ligase light chain subfamily.
Information by UniProt
- Gamma ECS regulatory subunit antibody
- Gamma-ECS regulatory subunit antibody
- Gamma-glutamylcysteine synthetase regulatory subunit antibody
Western blot - Anti-GCLM antibody (ab81445)
Western blot analysis of 40µg Mouse heart lysate (lanes 1 and 2) labeling GCLM with ab81445 at 1:1000. A HRP-conjugated anti-rabbit (1:10000) was used as the secondary antibody.
Western blot - GCLM antibody (ab81445)
Anti-GCLM antibody (ab81445) at 1 µg/ml (in 5% skim milk / PBS buffer) + MCF7 Cell Lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 31 kDa
Observed band size: 31 kDa
Gel Concentration: 12%
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-GCLM antibody(ab81445)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling GCLM with ab81445 at 2.5 µg/ml.
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