Overview

  • Product name
    Anti-GCLM antibody [EPR6667]
    See all GCLM primary antibodies
  • Description
    Rabbit monoclonal [EPR6667] to GCLM
  • Tested applications
    Suitable for: WB, IP, IHC-P, Flow Cytmore details
    Unsuitable for: ICC
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    corresponding to Human GCLM aa 50-150.

  • Positive control
    • HeLa, A673, PC12, A431, and K562 cell lysates; Human breast carcinoma tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Applications

Our Abpromise guarantee covers the use of ab126704 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 31 kDa.
IP 1/10 - 1/100.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for ICC.
  • Target

    • Tissue specificity
      In all tissues examined. Highest levels in skeletal muscle.
    • Pathway
      Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2.
    • Sequence similarities
      Belongs to the aldo/keto reductase family. Glutamate--cysteine ligase light chain subfamily.
    • Information by UniProt
    • Database links
    • Alternative names
      • Gamma ECS regulatory subunit antibody
      • Gamma-ECS regulatory subunit antibody
      • Gamma-glutamylcysteine synthetase regulatory subunit antibody
      • GCLM antibody
      • GCS light chain antibody
      • GLCLR antibody
      • Glutamate cysteine ligase regulatory subunit antibody
      • Glutamate--cysteine ligase modifier subunit antibody
      • Glutamate--cysteine ligase regulatory subunit antibody
      • GSC light chain antibody
      • GSH0_HUMAN antibody
      see all

    Images

    • Overlay histogram showing HeLa cells stained with ab126704 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab126704, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
    • ab126704, at 1/50 dilution, staining GCLM in Paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry.
    • All lanes : Anti-GCLM antibody [EPR6667] (ab126704) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : A673 cell lysate
      Lane 3 : PC12 cell lysate
      Lane 4 : A431 cell lysate
      Lane 5 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size : 31 kDa

    References

    This product has been referenced in:
    • Zhang J  et al. Comparisons of ethanol extracts of chinese propolis (poplar type) and poplar gums based on the antioxidant activities and molecular mechanism. Evid Based Complement Alternat Med 2015:307594 (2015). Mouse . Read more (PubMed: 25802536) »
    • Jain SK  et al. Hydrogen sulfide upregulates glutamate-cysteine ligase catalytic subunit, glutamate-cysteine ligase modifier subunit, and glutathione and inhibits interleukin-1ß secretion in monocytes exposed to high glucose levels. Metab Syndr Relat Disord 12:299-302 (2014). WB ; Human . Read more (PubMed: 24665821) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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