Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human GCLM aa 200-300.
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Our Abpromise guarantee covers the use of ab124827 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 30 kDa (predicted molecular weight: 31 kDa).|
|IP||1/10 - 1/100.|
|IHC-P||1/50 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|Flow Cyt||1/100 - 1/500.
ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||1/250 - 1/500.|
ab124827 immunoprecipitating GCLM. 10µg of cell lysate was incubated with primary antibody at a dilution of 1/50 and VeriBlot for IP secondary antibody (HRP) (ab131366) at a dilution of 1/10000.
Lane 1: K562 (human chronic myelogenous leukemia) whole cell lysate (10ug)
Lane 2: K562 (human chronic myelogenous leukemia) whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab124827 in K562 (human chronic myelogenous leukemia) whole cell lysate
ab124827 staining GCLM in HeLa (human cervix adenocarcinoma) cells by flow cytometry. Cells were fixed with 4% paraformaldehyde and the sample was incubated with the primary antibody at a dilution of 1/100. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.
Isoytype control: Rabbit monoclonal IgG (Black)
Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)
ab124827 staining GCLM in HeLa (human cervix adenocarcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 100% methanol. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody. ab7291 (1/1000) and ab150120 (1/1000) were used as counterstains for primary antibody ab124827 and secondary antibody ab150077 respectively. DAPI was used as a nuclear counterstain.
Diluting and blocking buffer: 5% NFDM /TBST
ab124827 staining GCLM in human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/250. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at 1/500.
Negative control 1: PBS in place of primary antibody.
Diluting and blocking buffer: 5% NFDM/TBST
ab124827 has not yet been referenced specifically in any publications.