Overview

  • Product name
  • Description
    Mouse monoclonal to GCM1
  • Tested applications
    Suitable for: WB, ELISA, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Opossum
  • Immunogen

    Recombinant fragment corresponding to amino acids 108-166 (QQRKRCPNCD GPLKLIPCRG HGGFPVTNFW RHDGRFIFFQ SKGEHDHPKP ETKLEAEA*) of Human GCM1 (NP_003634) linked to a proprietary tag.

  • Positive control
    • FHs 173 whole extract. HeLa cells.
  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.

Properties

Applications

Our Abpromise guarantee covers the use of ab88748 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 49 kDa.
ELISA Use at an assay dependent concentration.
ICC/IF Use a concentration of 10 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Transcription factor that is necessary for placental development. Binds to the trophoblast-specific element 2 (TSE2) of the aromatase gene enhancer.
  • Tissue specificity
    Placenta specific.
  • Sequence similarities
    Contains 1 GCM DNA-binding domain.
  • Post-translational
    modifications
    Polyubiquitinated in the presence of UBE2D2 and FBXW2 (in vitro).
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Chorion specific transcription factor GCMa antibody
    • Chorion-specific transcription factor GCMa antibody
    • GCM 1 antibody
    • GCM A antibody
    • GCM motif protein 1 antibody
    • Gcm1 antibody
    • GCM1_HUMAN antibody
    • Gcm1rs1 antibody
    • GCMA antibody
    • Gcmrs2 antibody
    • Glial cells missing homolog 1 antibody
    • Glial cells missing homolog a antibody
    • Glide antibody
    • hGCMa antibody
    see all

Images

  • Anti-GCM1 antibody (ab88748) at 5 µg/ml + FHs 173 WE cell lysate at 50 µg

    Predicted band size : 49 kDa
    Observed band size : ~52 kDa (why is the actual band size different from the predicted?)
  • ab88748 at 10 µg/ml staining GCM1 in paraformaldehyde-fixed, permeabilised HeLa cells by immunofluorescence.
  • ab88748 at 10 µg/ml detecting GCM1 by sandwich ELISA. The detection limit for GCM1, is approximately 0.03 ng/ml when using ab88748 as a capture antibody.
  • Overlay histogram showing JEG3 cells stained with ab88748 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab88748, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References

ab88748 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (12%)
Sample
Mouse Cell lysate - whole cell (Mouse trophoblast stem cell)
Specification
Mouse trophoblast stem cell
Treatment
various concentrations of X (a drug) for 1 h
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Dr. Hongfeng Ruan

Verified customer

Submitted Aug 19 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Sheep Tissue sections (Placentome)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium citrate
Permeabilization
No
Specification
Placentome
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative
Carnoy's fixative
Username

Miss. Barbara Makela

Verified customer

Submitted Oct 30 2015

Application
Western blot
Sample
Sheep Tissue lysate - whole (Placenta, caruncle)
Gel Running Conditions
Reduced Denaturing (10% PAG)
Loading amount
10 µg
Specification
Placenta, caruncle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Miss. Barbara Makela

Verified customer

Submitted Oct 30 2015

Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (placenta)
Permeabilization
No
Specification
placenta
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Aug 13 2015

Abreviews
Application
Western blot
Sample
Human Tissue lysate - whole (placenta)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
placenta
Blocking step
4% block from PE as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jul 08 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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