Overview

  • Product name
  • Description
    Mouse monoclonal to GCM1
  • Tested applications
    Suitable for: WB, ELISA, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Opossum
  • Immunogen

    Recombinant fragment corresponding to amino acids 108-166 (QQRKRCPNCD GPLKLIPCRG HGGFPVTNFW RHDGRFIFFQ SKGEHDHPKP ETKLEAEA*) of Human GCM1 (NP_003634) linked to a proprietary tag.

  • Positive control
    • FHs 173 whole extract. HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab88748 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 49 kDa.
ELISA Use at an assay dependent concentration.
ICC/IF Use a concentration of 10 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Transcription factor that is necessary for placental development. Binds to the trophoblast-specific element 2 (TSE2) of the aromatase gene enhancer.
  • Tissue specificity
    Placenta specific.
  • Sequence similarities
    Contains 1 GCM DNA-binding domain.
  • Post-translational
    modifications
    Polyubiquitinated in the presence of UBE2D2 and FBXW2 (in vitro).
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Chorion specific transcription factor GCMa antibody
    • Chorion-specific transcription factor GCMa antibody
    • GCM 1 antibody
    • GCM A antibody
    • GCM motif protein 1 antibody
    • Gcm1 antibody
    • GCM1_HUMAN antibody
    • Gcm1rs1 antibody
    • GCMA antibody
    • Gcmrs2 antibody
    • Glial cells missing homolog 1 antibody
    • Glial cells missing homolog a antibody
    • Glide antibody
    • hGCMa antibody
    see all

Anti-GCM1 antibody images

  • Anti-GCM1 antibody (ab88748) at 5 µg/ml + FHs 173 WE cell lysate at 50 µg

    Predicted band size : 49 kDa
    Observed band size : ~52 kDa (why is the actual band size different from the predicted?)
  • ab88748 at 10 µg/ml staining GCM1 in paraformaldehyde-fixed, permeabilised HeLa cells by immunofluorescence.
  • ab88748 at 10 µg/ml detecting GCM1 by sandwich ELISA. The detection limit for GCM1, is approximately 0.03 ng/ml when using ab88748 as a capture antibody.
  • Overlay histogram showing JEG3 cells stained with ab88748 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab88748, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-GCM1 antibody (ab88748)

ab88748 has not yet been referenced specifically in any publications.

Product Wall

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (12%)
Sample
Mouse Cell lysate - whole cell (Mouse trophoblast stem cell)
Specification
Mouse trophoblast stem cell
Treatment
various concentrations of X (a drug) for 1 h
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Dr. Hongfeng Ruan

Verified customer

Submitted Aug 19 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Sheep Tissue sections (Placentome)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium citrate
Permeabilization
No
Specification
Placentome
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative
Carnoy's fixative
Username

Mr. Jeremy Gingrich

Verified customer

Submitted Oct 30 2015

Application
Western blot
Sample
Sheep Tissue lysate - whole (Placenta, caruncle)
Gel Running Conditions
Reduced Denaturing (10% PAG)
Loading amount
10 µg
Specification
Placenta, caruncle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Mr. Jeremy Gingrich

Verified customer

Submitted Oct 30 2015

Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (placenta)
Permeabilization
No
Specification
placenta
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Aug 13 2015

Abreviews
Application
Western blot
Sample
Human Tissue lysate - whole (placenta)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
placenta
Blocking step
4% block from PE as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jul 08 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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