Overview

  • Product name
  • Description
    Rabbit polyclonal to GCM2
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus GCM2.

    (Peptide available as ab69686.)

  • Positive control
    • This antibody gave a positive signal in the following lysates: F9 whole cell Brain (Mouse) tissue Brain (Mouse) tissue, 0 days old Skeletal Muscle (Mouse) Tissue Thymus (Rat) Tissue

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab64723 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 56 kDa).

Target

  • Relevance
    Gcm2, a mouse ortholog of the Drosophila Glial Cells Missing gene, is expressed in the parathyroid-specific domains in the 3rd pouches from E9.5. The null mutation of Gcm2 causes aparathyroidism in the fetal and adult mouse and has been proposed to be a master regulator for parathyroid development. During Drosophila embryogenesis Gcm2 plays a crucial role in promoting glial cell differentiation.
  • Cellular localization
    Nuclear
  • Database links
  • Alternative names
    • chorion specific transcription factor GCMb antibody
    • Chorion-specific transcription factor GCMb antibody
    • GCM motif protein 2 antibody
    • GCMb antibody
    • Glial cells missing 2 antibody
    • glial cells missing homolog 2 (Drosophila) antibody
    • Glial cells missing homolog 2 antibody
    • glial cells missing homolog b antibody
    • glide gcm protein homolog antibody
    • glide/gcm protein homolog antibody
    • hGCMb antibody
    see all

Images

  • All lanes : Anti-GCM2 antibody (ab64723) at 1 µg/ml

    Lane 1 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate
    Lane 2 : Brain (Mouse) Tissue Lysate
    Lane 3 : Mouse brain tissue lysate - total protein (0 days) (ab7188)
    Lane 4 : Skeletal Muscle (Mouse) Tissue Lysate
    Lane 5 : Thymus (Rat) Tissue Lysate
    Lane 6 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate with Human GCM2 peptide (ab69686) at 1 µg/ml
    Lane 7 : Brain (Mouse) Tissue Lysate with Human GCM2 peptide (ab69686) at 1 µg/ml
    Lane 8 : Mouse brain tissue lysate - total protein (0 days) (ab7188) with Human GCM2 peptide (ab69686) at 1 µg/ml
    Lane 9 : Skeletal Muscle (Mouse) Tissue Lysate with Human GCM2 peptide (ab69686) at 1 µg/ml
    Lane 10 : Thymus (Rat) Tissue Lysate with Human GCM2 peptide (ab69686) at 1 µg/ml

    Lysates/proteins at 10 µg/ml per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 56 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 40 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes

References

This product has been referenced in:
  • Bain VE  et al. Tissue-specific roles for sonic hedgehog signaling in establishing thymus and parathyroid organ fate. Development 143:4027-4037 (2016). Read more (PubMed: 27633995) »
  • Reeh KA  et al. Ectopic TBX1 suppresses thymic epithelial cell differentiation and proliferation during thymus organogenesis. Development 141:2950-8 (2014). Read more (PubMed: 25053428) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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