All lanes : Anti-GCN2 antibody (ab70214) at 0.1 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg Lane 2 : Whole cell lysate from HeLa cells at 15 µg Lane 3 : Whole cell lysate from HeLa cells at 5 µg Lane 4 : Whole cell lysate from 293T cells at 50 µg Lane 5 : Whole cell lysate from NIH3T3 cells at 50 µg
Predicted band size : 187 kDa Observed band size : 187 kDa Additional bands at : 171 kDa,75 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 10 seconds
Immunoprecipitation - GCN2 antibody (ab70214)
Detection of Human GCN2 by Immunoprecipitation in Whole cell lysate from HeLa cells using ab70214 at 3 µg/mg lysate for IP and at 0.1 µg/ml for subsequent WB detection.
ICC/IF image of ab70214 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70214, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-GCN2 antibody (ab70214)
This product has been referenced in:
Tattoli I et al. Listeria phospholipases subvert host autophagic defenses by stalling pre-autophagosomal structures. EMBO J32:3066-78 (2013).
Read more (PubMed: 24162724) »
Kwon NH et al. Dual role of methionyl-tRNA synthetase in the regulation of translation and tumor suppressor activity of aminoacyl-tRNA synthetase-interacting multifunctional protein-3. Proc Natl Acad Sci U S A108:19635-40 (2011).
Read more (PubMed: 22106287) »