Overview

  • Product nameAnti-GCN2 antibody
    See all GCN2 primary antibodies
  • Description
    Rabbit polyclonal to GCN2
  • SpecificityWe have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • Tested applicationsSuitable for: ICC/IF, WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Horse, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between N terminal residues 1 and 50 of human GCN2. (NP_001013725.2).

  • Positive control
    • Whole cell lysate from HeLa cells

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab70214 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/5000 - 1/15000. Detects a band of approximately 187 kDa (predicted molecular weight: 187 kDa).
IP Use at 2 µg/mg of lysate.

Target

  • FunctionCan phosphorylate the alpha subunit of EIF2 and may mediate translational control.
  • Tissue specificityWidely expressed.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.
    Contains 2 protein kinase domains.
    Contains 1 RWD domain.
  • DomainKinase domain 1 is a degenerate kinase domain.
    RWD domain is reported to interact with GCN1L1.
  • Post-translational
    modifications
    Autophosphorylated on threonine residues.
  • Information by UniProt
  • Database links
  • Alternative names
    • E2AK4_HUMAN antibody
    • Eif2ak4 antibody
    • Eukaryotic Translation Initiation Factor 2 alpha kinase 4 antibody
    • Eukaryotic translation initiation factor 2-alpha kinase 4 antibody
    • GCN2 antibody
    • GCN2 eIF2alpha kinase antibody
    • GCN2 like protein antibody
    • GCN2-like protein antibody
    • KIAA1338 antibody
    • MGCN2 antibody
    see all

Anti-GCN2 antibody images

  • All lanes : Anti-GCN2 antibody (ab70214) at 0.1 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg
    Lane 5 : Whole cell lysate from NIH3T3 cells at 50 µg


    Predicted band size : 187 kDa
    Observed band size : 187 kDa
    Additional bands at : 171 kDa,75 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 10 seconds
  • Detection of Human GCN2 by Immunoprecipitation in Whole cell lysate from HeLa cells using ab70214 at 3 µg/mg lysate for IP and at 0.1 µg/ml for subsequent WB detection.
  • ICC/IF image of ab70214 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70214, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-GCN2 antibody (ab70214)

This product has been referenced in:
  • Tattoli I  et al. Listeria phospholipases subvert host autophagic defenses by stalling pre-autophagosomal structures. EMBO J 32:3066-78 (2013). Read more (PubMed: 24162724) »
  • Kwon NH  et al. Dual role of methionyl-tRNA synthetase in the regulation of translation and tumor suppressor activity of aminoacyl-tRNA synthetase-interacting multifunctional protein-3. Proc Natl Acad Sci U S A 108:19635-40 (2011). WB ; Human . Read more (PubMed: 22106287) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"