Anti-GCN2 (phospho T667) antibody [EPR2319Y] (ab68427)

Overview

  • Product nameAnti-GCN2 (phospho T667) antibody [EPR2319Y]
    See all GCN2 primary antibodies
  • Description
    Rabbit monoclonal [EPR2319Y] to GCN2 (phospho T667)
  • SpecificityDetects GCN2 only when phosphorylyated at threonine 667.
  • Tested applicationsSuitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic phosphopeptide corresponding to residues surrounding threonine 667 of human GCN2.

  • Positive control
    • HeLa cells
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab68427 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 220 kDa (predicted molecular weight: 220 kDa).
IP 1/30.
  • Application notesIs unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • FunctionCan phosphorylate the alpha subunit of EIF2 and may mediate translational control.
    • Tissue specificityWidely expressed.
    • Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.
      Contains 2 protein kinase domains.
      Contains 1 RWD domain.
    • DomainKinase domain 1 is a degenerate kinase domain.
      RWD domain is reported to interact with GCN1L1.
    • Post-translational
      modifications
      Autophosphorylated on threonine residues.
    • Information by UniProt
    • Database links
    • Alternative names
      • E2AK4_HUMAN antibody
      • Eif2ak4 antibody
      • Eukaryotic Translation Initiation Factor 2 alpha kinase 4 antibody
      • Eukaryotic translation initiation factor 2-alpha kinase 4 antibody
      • GCN2 antibody
      • GCN2 eIF2alpha kinase antibody
      • GCN2 like protein antibody
      • GCN2-like protein antibody
      • KIAA1338 antibody
      • MGCN2 antibody
      see all

    Anti-GCN2 (phospho T667) antibody [EPR2319Y] images

    • All lanes : Anti-GCN2 (phospho T667) antibody [EPR2319Y] (ab68427) at 1/2000 dilution

      Lane 1 : HeLa cell lysate - untreated
      Lane 2 : HeLa cell lysate - treated with LP

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled Goat anti-Rabbit antibody at 1/2000 dilution

      Predicted band size : 220 kDa
      Observed band size : 220 kDa
      Additional bands at : 180 kDa. We are unsure as to the identity of these extra bands.

    References for Anti-GCN2 (phospho T667) antibody [EPR2319Y] (ab68427)

    ab68427 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"