Recombinant
RabMAb

Anti-GCN2 (phospho T667) antibody [EPR2319Y] (ab68427)

Overview

  • Product name
    Anti-GCN2 (phospho T667) antibody [EPR2319Y]
    See all GCN2 primary antibodies
  • Description
    Rabbit monoclonal [EPR2319Y] to GCN2 (phospho T667)
  • Specificity
    Detects GCN2 only when phosphorylyated at threonine 667.
  • Tested applications
    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic phosphopeptide corresponding to residues surrounding threonine 667 of human GCN2.

  • Positive control
    • HeLa cells
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab68427 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 220 kDa (predicted molecular weight: 220 kDa).
IP 1/30.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Function
      Can phosphorylate the alpha subunit of EIF2 and may mediate translational control.
    • Tissue specificity
      Widely expressed.
    • Sequence similarities
      Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.
      Contains 2 protein kinase domains.
      Contains 1 RWD domain.
    • Domain
      Kinase domain 1 is a degenerate kinase domain.
      RWD domain is reported to interact with GCN1L1.
    • Post-translational
      modifications
      Autophosphorylated on threonine residues.
    • Information by UniProt
    • Database links
    • Alternative names
      • E2AK4_HUMAN antibody
      • Eif2ak4 antibody
      • Eukaryotic Translation Initiation Factor 2 alpha kinase 4 antibody
      • Eukaryotic translation initiation factor 2-alpha kinase 4 antibody
      • GCN2 antibody
      • GCN2 eIF2alpha kinase antibody
      • GCN2 like protein antibody
      • GCN2-like protein antibody
      • KIAA1338 antibody
      • MGCN2 antibody
      see all

    Images

    • All lanes : Anti-GCN2 (phospho T667) antibody [EPR2319Y] (ab68427) at 1/2000 dilution

      Lane 1 : HeLa cell lysate - untreated
      Lane 2 : HeLa cell lysate - treated with LP

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled Goat anti-Rabbit antibody at 1/2000 dilution

      Predicted band size : 220 kDa
      Observed band size : 220 kDa
      Additional bands at : 180 kDa. We are unsure as to the identity of these extra bands.

    References

    ab68427 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab68427.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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