Anti-GCN2 (phospho T899) antibody [EPR2320Y] (ab75836)

Overview

  • Product nameAnti-GCN2 (phospho T899) antibody [EPR2320Y]
    See all GCN2 primary antibodies
  • Description
    Rabbit monoclonal [EPR2320Y] to GCN2 (phospho T899)
  • SpecificityThis antibody detects GCN2 phosphorylated at threonine 899.
  • Tested applicationsSuitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human GCN2 (phospho T899).

  • Positive control
    • WB: HeLa cell lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab75836 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 220 kDa (predicted molecular weight: 187 kDa).

For unpurified use at 1/500.

  • Application notesIs unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • FunctionCan phosphorylate the alpha subunit of EIF2 and may mediate translational control.
    • Tissue specificityWidely expressed.
    • Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.
      Contains 2 protein kinase domains.
      Contains 1 RWD domain.
    • DomainKinase domain 1 is a degenerate kinase domain.
      RWD domain is reported to interact with GCN1L1.
    • Post-translational
      modifications
      Autophosphorylated on threonine residues.
    • Information by UniProt
    • Database links
    • Alternative names
      • E2AK4_HUMAN antibody
      • Eif2ak4 antibody
      • Eukaryotic Translation Initiation Factor 2 alpha kinase 4 antibody
      • Eukaryotic translation initiation factor 2-alpha kinase 4 antibody
      • GCN2 antibody
      • GCN2 eIF2alpha kinase antibody
      • GCN2 like protein antibody
      • GCN2-like protein antibody
      • KIAA1338 antibody
      • MGCN2 antibody
      see all

    Anti-GCN2 (phospho T899) antibody [EPR2320Y] images

    • All lanes : Anti-GCN2 (phospho T899) antibody [EPR2320Y] (ab75836) at 1/1000 dilution (purified)

      Lane 1 : HeLa cell lysate - untreated
      Lane 2 : HeLa cell lysate - treated with Calyculin

      Lysates/proteins at 10 µg per lane.

      Secondary
      Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size : 187 kDa
      Observed band size : 220 kDa (why is the actual band size different from the predicted?)

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • All lanes : Anti-GCN2 (phospho T899) antibody [EPR2320Y] (ab75836) at 1/500 dilution (unpurified)

      Lane 1 : HeLa cell lysate - untreated
      Lane 2 : HeLa cell lysate - treated with Calyculin A

      Lysates/proteins at 10 µg per lane.

      Secondary
      Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size : 187 kDa
      Observed band size : 220 kDa (why is the actual band size different from the predicted?)

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    References for Anti-GCN2 (phospho T899) antibody [EPR2320Y] (ab75836)

    This product has been referenced in:
    • Chaveroux C  et al. In vivo imaging of the spatiotemporal activity of the eIF2a-ATF4 signaling pathway: Insights into stress and related disorders. Sci Signal 8:rs5 (2015). WB ; Mouse . Read more (PubMed: 25921292) »
    • Wengrod J  et al. Phosphorylation of eIF2a triggered by mTORC1 inhibition and PP6C activation is required for autophagy and is aberrant in PP6C-mutated melanoma. Sci Signal 8:ra27 (2015). WB ; Human . Read more (PubMed: 25759478) »

    See all 8 Publications for this product

    Product Wall

    Application Western blot
    Sample Mouse Cell lysate - whole cell (C57BL/6)
    Gel Running Conditions Reduced Denaturing (8%, 50V for 30 min, 150V for 145 min)
    Loading amount 17.8 µg
    Treatment UV radiation, WT mouse adenovirus type 1, or amino acid deficiency
    Specification C57BL/6
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Username

    Abcam user community

    Verified customer

    Submitted Jan 19 2016

    Tris acetate gel plus Tris acetate running buffer is the usual recommendation for large proteins.You will get results with those other buffering systems but I cannot say for sure they will improve upon the result you had with Tris-acetate. The laborato...

    Read More

    Vielen Dank für Ihre Anfrage.


    Leider bieten wir generell keine kostenlosen Proben zu Testzwecken an, da wir bei über 90.000Produkten gar nicht die logistischen Möglichkeiten haben, neben unserer eigentlichen Produktpallette...

    Read More

    Thank you for contacting us and your interest in our products.

    The sequence homology between the immunogen used to raise the anti-GCN2 (phospho T899) antibody (ab75836) and the mouse protein are very high, and you would therefore expect the a...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Cell lysate - whole cell (MEF (Mouse Embryonic Fibroblasts))
    Loading amount 20 µg
    Specification MEF (Mouse Embryonic Fibroblasts)
    Treatment L-Leucine starvation for 1, 3 and 6 hrs
    Gel Running Conditions Reduced Denaturing (6%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Mr. Martin Roffe

    Verified customer

    Submitted Apr 07 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"