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Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human GCNF.
(Peptide available as ab38815.)
Our Abpromise guarantee covers the use of ab38816 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/250. Detects a band of approximately 53 kDa (predicted molecular weight: 54 kDa).Can be blocked with Human GCNF peptide (ab38815).|
|IHC-P||Use at an assay dependent concentration. PubMed: 22926843|
IHC-P image of GCNF staining with ab38816 on tissue sections from adult mouse testis. The sections were subjected to heat-mediated antigen retrieval using Dako antigen retrieval solution. The sections were then blocked with 5% milk for 30 minutes at 25°C, before incubation with ab38816 (1/100 dilution) for 18 hours at 4°C. The secondary was an Alexa-Fluor 555 conjugated goat anti-rabbit polyclonal, used at a 1/500 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"