Overview

  • Product nameAnti-GCNT1 antibody
    See all GCNT1 primary antibodies
  • Description
    Rabbit polyclonal to GCNT1
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-428 of Human GCNT1 (NP_001481.2).

  • Positive control
    • Human Pancreas Lysate and transfected 293T lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab102665 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 50 kDa.
ICC/IF 1/50.

Target

  • FunctionForms critical branches in O-glycans.
  • Tissue specificityHighly expressed in activated T-lymphocytes and myeloid cells.
  • PathwayProtein modification; protein glycosylation.
  • Sequence similaritiesBelongs to the glycosyltransferase 14 family.
  • Cellular localizationGolgi apparatus membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 3-galactosyl-O-glycosyl-glycoprotein beta-1 antibody
    • 6-N-acetylglucosaminyltransferase antibody
    • Beta-1 antibody
    • Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase antibody
    • C2GNT antibody
    • C2GNT L antibody
    • C2GNT1 antibody
    • C2GnTL antibody
    • Core 2 branching enzyme antibody
    • Core 2 GNT antibody
    • Core 2-branching enzyme antibody
    • Core2 GlcNAc transferase antibody
    • Core2-GlcNAc-transferase antibody
    • GCNT 1 antibody
    • GCNT1 antibody
    • GCNT1_HUMAN antibody
    • glucosaminyl (N-acetyl) transferase 1 core 2 antibody
    • NACGT2 antibody
    see all

Anti-GCNT1 antibody images

  • Anti-GCNT1 antibody (ab102665) at 1/500 dilution + Human Pancreas Tissue Lysate at 50 µg

    Predicted band size : 50 kDa
  • All lanes : Anti-GCNT1 antibody (ab102665) at 1/500 dilution

    Lane 1 : GCNT1 transfected 293T cell line
    Lane 2 : Non-transfected 293T cell line

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 50 kDa

References for Anti-GCNT1 antibody (ab102665)

This product has been referenced in:
  • Nolz JC & Harty JT IL-15 regulates memory CD8+ T cell O-glycan synthesis and affects trafficking. J Clin Invest 124:1013-26 (2014). WB ; Mouse . Read more (PubMed: 24509081) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Cell (Mouse tracheal tissue)
Permeabilization Yes - 0.2% Triton X-100
Specification Mouse tracheal tissue
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative Formaldehyde
Username

Dr. Armen Petrosyan

Verified customer

Submitted Jan 20 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (KG1a)
Specification KG1a
Fixative Formaldehyde
Permeabilization Yes - 0.2% saponin
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Username

Dr. Armen Petrosyan

Verified customer

Submitted Apr 26 2012

DISCOUNT CODE: ***
Expiration date: August 25, 2012

This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for ab102665 in ICC/IFand include this code in the “Add...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"