Anti-GCNT2 antibody (ab107511)

Overview

  • Product nameAnti-GCNT2 antibody
    See all GCNT2 primary antibodies
  • Description
    Rabbit polyclonal to GCNT2
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to C terminal amino acids 371-399 of Human GCNT2 (NP_001482.1).

  • Positive control
    • Mouse kidney tissue lysate. Formalin fixed and paraffin embedded Human prostate carcinoma

Properties

Applications

Our Abpromise guarantee covers the use of ab107511 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 46 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • RelevanceGCNT2 is the I-branching enzyme, a beta-1,6-N-acetylglucosaminyltransferase that converts linear into branched poly-N-acetyllactosaminoglycans and is responsible for the conversion of fetal i antigen to adult I antigen in erythrocytes during embryonic development. Mutations in this gene have been associated with adult i blood group phenotype. Alternatively spliced transcript variants encoding 3 different isoforms have been described.
  • Cellular localizationGolgi apparatus membrane.
  • Database links
  • Alternative names
    • bA360O19.2 antibody
    • bA421M1.1 antibody
    • Beta 1 6 N acetylglucosaminyltransferase 2 antibody
    • CCAT antibody
    • GCNT 2 antibody
    • GCNT2C antibody
    • GCNT5 antibody
    • Glucosaminyl (N acetyl) transferase 2 I branching enzyme (I blood group) antibody
    • Glucosaminyl (N acetyl) transferase 2 I branching enzyme antibody
    • I beta 1 6 N acetylglucosaminyltransferase antibody
    • I branching beta 1 6 acetylglucosaminyltransferase antibody
    • I branching enzyme antibody
    • IGNT antibody
    • II antibody
    • Ii blood group antibody
    • MGC163396 antibody
    • N acetylglucosaminyltransferase antibody
    • N acetyllactosaminide beta 1 6 N acetylglucosaminyltransferase antibody
    • N acetyllactosaminide beta 1,6 N acetylglucosaminyl transferase antibody
    • NACGT1 antibody
    • NAGCT1 antibody
    • ULG3 antibody
    see all

Anti-GCNT2 antibody images

  • Anti-GCNT2 antibody (ab107511) at 1/100 dilution + Mouse kidney tissue lysate at 35 µg

    Predicted band size : 46 kDa
  • ab107511, at 1/50, staining GCNT2 in formalin fixed and paraffin embedded Human prostate carcinoma by Immunohistochemistry.

References for Anti-GCNT2 antibody (ab107511)

ab107511 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (SU.86.86 (pancreatic carcinoma - liver metastasis))
Permeabilization Yes - 0.2% Triton X-100
Specification SU.86.86 (pancreatic carcinoma - liver metastasis)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative Formaldehyde
Username

Dr. Armen Petrosyan

Verified customer

Submitted Jul 27 2015

Thank you very much for your reply.

I agree that the UniProt information is unexpected,so I checked the publications cited for the tissue specificity information. Neither publication mentions placenta so I'm not sure where this information ca...

Read More

Thank you very much for your reply and for keeping me updated with the results.

Based oninformation from the Human Protein Atlas and UniProt, prostate, small intestine, and colon seem to be good positive controls-

http://www.protein...

Read More

Thank you very much for your email.

I apologize that I neglected to include this information yesterday. The replacement antibody ab69964 was tested with 5% milk in PBSTblocking, so I would recommend using that same blocking buffer. The antibo...

Read More

Thank you very much for your reply and for the additional information.

I agree that trying another antibody would be a good idea, and I'm sending a free of charge vial of ab69964 on the order *** which should arrive tomorrow.

Please...

Read More

Thank you very much for your reply and for sending these images.

I was not able to open the second image following BSA blocking, but I've reviewed the original image and some information about the protein. I'm wondering what the correlation i...

Read More

Thank you very much for your call today and for letting us know about the trouble with ab107511.

As we discussed, please send the images of the Western blots once they are available, and I'll be happy to see if there are any other suggestions...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"