Overview

  • Product name
  • Description
    Rabbit polyclonal to GCNT3
  • Tested applications
    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide derived from an internal sequence of Human GCNT3.

  • Positive control
    • A549 cells and Human liver carcinoma tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49.1% PBS, 50% Glycerol, 0.88% Sodium chloride
    Note: PBS (without Mg2+ and Ca2+)
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab111371 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab111371 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/500.

Target

  • Function
    Glycosyltransferase that can synthesize all known mucin beta 6 N-acetylglucosaminides. Mediates core 2 and core 4 O-glycan branching, 2 important steps in mucin-type biosynthesis. Has also I-branching enzyme activity by converting linear into branched poly-N-acetyllactosaminoglycans, leading to introduce the blood group I antigen during embryonic development.
  • Tissue specificity
    Primarily expressed in mucus-secreting tissues. Expressed in colon, kidney, small intestine, trachea, and stomach, where mucin is produced.
  • Pathway
    Protein modification; protein glycosylation.
  • Sequence similarities
    Belongs to the glycosyltransferase 14 family.
  • Post-translational
    modifications
    N-glycosylated.
  • Cellular localization
    Golgi apparatus membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2010013H22Rik antibody
    • 2210021I22Rik antibody
    • 2210401J11Rik antibody
    • 3-galactosyl-O-glycosyl-glycoprotein beta-1 antibody
    • 6-N-acetylglucosaminyltransferase 3 antibody
    • 6-N-acetylglucosaminyltransferase antibody
    • Beta 1 3 galactosyl O glycosyl glycoprotein beta 1 6 N acetylglucosaminyltransferase 3 antibody
    • Beta-1 antibody
    • Beta1 6 N acetylglucosaminyltransferase 3 antibody
    • beta1 6 N acetylglucosaminyltransferase antibody
    • C2/4GnT antibody
    • C24GNT antibody
    • C2GnT M antibody
    • C2GnT mucin type antibody
    • C2GnT-M antibody
    • C2GnT-mucin type antibody
    • C2GnT2 antibody
    • C2GNTM antibody
    • Core 2 beta 1 6 N acetylglucosaminyltransferase II antibody
    • Core 2/core 4 beta 1 6 N acetylglucosaminyltransferase antibody
    • Core 2/core 4 beta-1 antibody
    • dI/C2/C4GnT antibody
    • EC 2.4.1.102 antibody
    • EC 2.4.1.150 antibody
    • GCNT3 antibody
    • GCNT3_HUMAN antibody
    • Glucosaminyl (N acetyl) transferase 3 antibody
    • Glucosaminyl (N acetyl) transferase 3 mucin type antibody
    • glucosaminyl (N-acetyl) transferase 3, mucin type antibody
    • GnT M antibody
    • GNTM antibody
    • hC2GnT M antibody
    • hC2GnT-M antibody
    • Mucus-type core 2 beta-1,6-N-acetylglucosaminyltransferase antibody
    • OTTHUMP00000163601 antibody
    see all

Images

  • ab111371 at 1/100 dilution staining GCNT3 in A549 cells by Immunofluorescence. The image on the right is treated with the synthesized peptide.
  • ab111371 at 1/50 dilution staining GCNT3 in paraffin-embedded Human Liver carcinoma tissue by Immunohistochemistry. The image on the right is treated with the synthesized peptide.

References

ab111371 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Tumor)
Permeabilization
Yes - Triton X100
Specification
Tumor
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde
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Verified customer

Submitted Feb 09 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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