Overview

  • Product nameAnti-GCNT3 antibody
    See all GCNT3 primary antibodies
  • Description
    Rabbit polyclonal to GCNT3
  • Tested applicationsSuitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide derived from an internal sequence of Human GCNT3.

  • Positive control
    • A549 cells and Human liver carcinoma tissue.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferpH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49.1% PBS, 50% Glycerol, 0.88% Sodium chloride
    Note: PBS (without Mg2+ and Ca2+)
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab111371 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab111371 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/500.

Target

  • FunctionGlycosyltransferase that can synthesize all known mucin beta 6 N-acetylglucosaminides. Mediates core 2 and core 4 O-glycan branching, 2 important steps in mucin-type biosynthesis. Has also I-branching enzyme activity by converting linear into branched poly-N-acetyllactosaminoglycans, leading to introduce the blood group I antigen during embryonic development.
  • Tissue specificityPrimarily expressed in mucus-secreting tissues. Expressed in colon, kidney, small intestine, trachea, and stomach, where mucin is produced.
  • PathwayProtein modification; protein glycosylation.
  • Sequence similaritiesBelongs to the glycosyltransferase 14 family.
  • Post-translational
    modifications
    N-glycosylated.
  • Cellular localizationGolgi apparatus membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2010013H22Rik antibody
    • 2210021I22Rik antibody
    • 2210401J11Rik antibody
    • 3-galactosyl-O-glycosyl-glycoprotein beta-1 antibody
    • 6-N-acetylglucosaminyltransferase 3 antibody
    • 6-N-acetylglucosaminyltransferase antibody
    • Beta 1 3 galactosyl O glycosyl glycoprotein beta 1 6 N acetylglucosaminyltransferase 3 antibody
    • Beta-1 antibody
    • Beta1 6 N acetylglucosaminyltransferase 3 antibody
    • beta1 6 N acetylglucosaminyltransferase antibody
    • C2/4GnT antibody
    • C24GNT antibody
    • C2GnT M antibody
    • C2GnT mucin type antibody
    • C2GnT-M antibody
    • C2GnT-mucin type antibody
    • C2GnT2 antibody
    • C2GNTM antibody
    • Core 2 beta 1 6 N acetylglucosaminyltransferase II antibody
    • Core 2/core 4 beta 1 6 N acetylglucosaminyltransferase antibody
    • Core 2/core 4 beta-1 antibody
    • dI/C2/C4GnT antibody
    • EC 2.4.1.102 antibody
    • EC 2.4.1.150 antibody
    • GCNT3 antibody
    • GCNT3_HUMAN antibody
    • Glucosaminyl (N acetyl) transferase 3 antibody
    • Glucosaminyl (N acetyl) transferase 3 mucin type antibody
    • glucosaminyl (N-acetyl) transferase 3, mucin type antibody
    • GnT M antibody
    • GNTM antibody
    • hC2GnT M antibody
    • hC2GnT-M antibody
    • Mucus-type core 2 beta-1,6-N-acetylglucosaminyltransferase antibody
    • OTTHUMP00000163601 antibody
    see all

Anti-GCNT3 antibody images

  • ab111371 at 1/100 dilution staining GCNT3 in A549 cells by Immunofluorescence. The image on the right is treated with the synthesized peptide.
  • ab111371 at 1/50 dilution staining GCNT3 in paraffin-embedded Human Liver carcinoma tissue by Immunohistochemistry. The image on the right is treated with the synthesized peptide.

References for Anti-GCNT3 antibody (ab111371)

ab111371 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Tumor)
Permeabilization Yes - Triton X100
Specification Tumor
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Feb 09 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"