Anti-GCNT3 antibody (ab77728)
Key features and details
- Goat polyclonal to GCNT3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GCNT3 antibody
See all GCNT3 primary antibodies -
Description
Goat polyclonal to GCNT3 -
Host species
Goat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Horse, Dog, Xenopus laevis, Chimpanzee, Rhesus monkey -
Immunogen
Synthetic peptide:
C-NKFDPKVDDNALQ
, corresponding to internal sequence amino acids 410-422 of Human GCNT3 (NP_004742.1). -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab77728 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab77728 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Notes |
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WB: Use at a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 51 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Glycosyltransferase that can synthesize all known mucin beta 6 N-acetylglucosaminides. Mediates core 2 and core 4 O-glycan branching, 2 important steps in mucin-type biosynthesis. Has also I-branching enzyme activity by converting linear into branched poly-N-acetyllactosaminoglycans, leading to introduce the blood group I antigen during embryonic development. -
Tissue specificity
Primarily expressed in mucus-secreting tissues. Expressed in colon, kidney, small intestine, trachea, and stomach, where mucin is produced. -
Pathway
Protein modification; protein glycosylation. -
Sequence similarities
Belongs to the glycosyltransferase 14 family. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Golgi apparatus membrane. - Information by UniProt
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Database links
- Entrez Gene: 487578 Dog
- Entrez Gene: 100068109 Horse
- Entrez Gene: 9245 Human
- Entrez Gene: 72077 Mouse
- Entrez Gene: 286976 Rat
- Entrez Gene: 495681 Xenopus laevis
- Omim: 606836 Human
- SwissProt: O95395 Human
see all -
Alternative names
- 2010013H22Rik antibody
- 2210021I22Rik antibody
- 2210401J11Rik antibody
see all
Images
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Anti-GCNT3 antibody (ab77728) at 0.1 µg/ml + Human Duodenum lysate (in RIPA buffer) at 35 µg
Predicted band size: 51 kDa
Observed band size: 57 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour.
Datasheets and documents
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Datasheet download
References (1)
ab77728 has been referenced in 1 publication.
- Li RW et al. The vitamin D receptor and inducible nitric oxide synthase associated pathways in acquired resistance to Cooperia oncophora infection in cattle. Vet Res 42:48 (2011). WB ; Cow . PubMed: 21414188