The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 52 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
FunctionPlays a role in cellular signaling via Rho-related GTP-binding proteins and subsequent activation of transcription factor SRF (By similarity). Targets TJP1 to cell junctions. In cortical neurons, may play a role in glutaminergic signal transduction through interaction with the NMDA receptor subunit GRIN1.
Tissue specificityDetected in heart, liver, skeletal muscle, placenta, small intestine, lung, prostate and testis.
Sequence similaritiesBelongs to the MYZAP family.
Cellular localizationCytoplasm > cytoskeleton. Cell membrane. Cytoplasm > myofibril > sarcomere > I band. Cytoplasm > myofibril > sarcomere > Z line. Cell junction. Detected predominantly at the intercalated disk in cardiomyocytes, and at low levels on sarcomeric Z disks. Colocalizes with F-actin. Colocalizes with cortical actin.