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Our Abpromise guarantee covers the use of ab9923 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent dilution. To detect hGCP-2 by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antibody, in conjunction with compatible secondary reagents, allows detection of 0.2 - 0.4 ng/well of recombinant hGCP-2.|
|Neutralising||Use at an assay dependent dilution. To yield one-half maximal inhibition [ND50] of the biological activity of hGCP-2 (100.0 ng/ml), a concentration of 2.0 – 5.0 µg/ml of this antibody is required.|
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hGCP-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.|