Overview

  • Product name
    Anti-GCP4 antibody
  • Description
    Rabbit polyclonal to GCP4
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rabbit, Cow, Xenopus laevis, Zebrafish, Xenopus tropicalis
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 99-314 of Human GCP4 (UniProt: Q9UGJ1).

  • Positive control
    • NIH 3T3 whole cell lysate; 293T cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab153939 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 76 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Relevance
    GCP4 is a component of the gamma-tubulin complex which is necessary for microtubule nucleation at the centrosome. GCP4 shows sequence identity with the C-terminal regions of human centrosomal proteins 103p (TUBGCP2) and 104p (TUBGCP3).
  • Cellular localization
    Centrosome.
  • Database links
  • Alternative names
    • 76P antibody
    • FLJ14797 antibody
    • Gamma tubulin complex component 4 antibody
    • Gamma tubulin ring complex protein (76p gene) antibody
    • Gamma tubulin ring complex protein antibody
    • Gamma-ring complex protein 76 kDa antibody
    • GCP 4 antibody
    • GCP4 antibody
    • Grip76 antibody
    • h76p antibody
    • hGCP4 antibody
    • Hgrip76 antibody
    • TUBGCP 4 antibody
    • TUBGCP4 antibody
    • Tubulin gamma complex associated protein 4 antibody
    see all

Images

  • Anti-GCP4 antibody (ab153939) at 1/1000 dilution + NIH 3T3 whole cell lysate at 30 µg

    Predicted band size: 76 kDa



    10 % SDS-PAGE
  • Confocal Immunofluorescent analysis of methanol-fixed 293T cells labeling GCP4 with ab153939 at 1/500 dilution (green). Alpha-tubulin filaments are labeled in red
  • Confocal Immunofluorescent analysis of paraformaldehyde-fixed 293T cells labeling GCP4 with ab153939 at 1/500 dilution (green). Alpha-tubulin filaments are labeled in red

References

ab153939 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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