Overview

  • Product name
  • Description
    Rabbit polyclonal to GCS1
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 144-193 (GPYGWEFHDG LSFGRQHIQD GALRLTTEFV KRPGGQHGGD WSWRVTVEPQ) of human GCS1 (NP_006293).

  • Positive control
    • HeLa cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab82962 is purified by a peptide affinity chromatography method.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab82962 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 92 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay, 1:312500.

Target

  • Function
    Cleaves the distal alpha 1,2-linked glucose residue from the Glc(3)Man(9)GlcNAc(2) oligosaccharide precursor in a highly specific manner.
  • Pathway
    Glycan metabolism; N-glycan degradation.
  • Involvement in disease
    Defects in MOGS are the cause of type IIb congenital disorder of glycosylation (CDGIIb) [MIM:606056]; also known as glucosidase I deficiency. CDGIIb is characterized by marked generalized hypotonia and hypomotility of the neonate, dysmorphic features, including a prominent occiput, short palpebral fissures, retrognathia, high arched palate, generalized edema, and hypoplastic genitalia. Symptoms of the infant included hepatomegaly, hypoventilation, feeding problems and seizures. The clinical course was progressive and the infant did not survive more than a few months.
  • Sequence similarities
    Belongs to the glycosyl hydrolase 63 family.
  • Cellular localization
    Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • EC 3.2.1.106 antibody
    • glucosidase I antibody
    • Mannosyl oligosaccharide glucosidase antibody
    • Mannosyl-oligosaccharide glucosidase antibody
    • Mogs antibody
    • MOGS_HUMAN antibody
    • Processing A glucosidase I antibody
    • Processing A-glucosidase I antibody
    see all

Anti-GCS1 antibody images

  • Anti-GCS1 antibody (ab82962) at 1 µg/ml + HeLa cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 92 kDa
    Observed band size : 92 kDa
    Additional bands at : 24 kDa,50 kDa. We are unsure as to the identity of these extra bands.

References for Anti-GCS1 antibody (ab82962)

ab82962 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab82962.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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