Overview

  • Product name
    Anti-GCSF Receptor antibody
    See all GCSF Receptor primary antibodies
  • Description
    Rabbit polyclonal to GCSF Receptor
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to a region within internal sequence amino acids 164-392 of Human GCSF Receptor (UniProt Q99062).

  • Positive control
    • CL1-5 xenograft; A431 cells; A431 whole cell lysate; 293T and Raji

Properties

Applications

Our Abpromise guarantee covers the use of ab126167 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 92 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/500.

Target

  • Function
    Receptor for granulocyte colony-stimulating factor (CSF3), essential for granulocytic maturation. Plays a crucial role in the proliferation, differientation and survival of cells along the neutrophilic lineage. In addition it may function in some adhesion or recognition events at the cell surface.
  • Tissue specificity
    One or several isoforms have been found in myelogenous leukemia cell line KG-1, leukemia U-937 cell line, in bone marrow cells, placenta, and peripheral blood granulocytes. Isoform GCSFR-2 is found only in leukemia U-937 cells. Isoform GCSFR-3 is highly expressed in placenta.
  • Involvement in disease
    Hereditary neutrophilia
  • Sequence similarities
    Belongs to the type I cytokine receptor family. Type 2 subfamily.
    Contains 5 fibronectin type-III domains.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
  • Domain
    The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    The box 1 motif is required for JAK interaction and/or activation.
  • Cellular localization
    Secreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 114 antibody
    • CD114 antibody
    • CD114 antigen antibody
    • Colony stimulating factor 3 receptor (granulocyte) antibody
    • Colony stimulating factor 3 receptor antibody
    • CSF 3R antibody
    • CSF3R antibody
    • CSF3R_HUMAN antibody
    • Csfgr antibody
    • G CSF R antibody
    • G-CSF receptor antibody
    • G-CSF-R antibody
    • GCSFR antibody
    • Granulocyte colony stimulating factor receptor antibody
    • Granulocyte colony-stimulating factor receptor antibody
    • OTTHUMP00000009703 antibody
    • OTTHUMP00000009704 antibody
    • OTTHUMP00000009705 antibody
    see all

Images

  • Anti-GCSF Receptor antibody (ab126167) at 1/1000 dilution + A431 whole cell lysate at 30 µg

    Predicted band size : 92 kDa
  • ab126167, at 1/500 dilution, staining GCSF Receptor in methanol-fixed A431 by Immunonfluorescence. The lower image is co-stained with Hoechst 33342.
  • ab126167, at 1/250 dilution, staining GCSF Receptor in paraffin-embedded CL1-5 xenograft by Immunohistochemistry.

References

This product has been referenced in:
  • Maxson JE  et al. The Colony-Stimulating Factor 3 Receptor T640N Mutation Is Oncogenic, Sensitive to JAK Inhibition, and Mimics T618I. Clin Cancer Res 22:757-64 (2016). WB ; Human . Read more (PubMed: 26475333) »
  • Maxson JE  et al. Ligand independence of the T618I mutation in the colony-stimulating factor 3 receptor (CSF3R) protein results from loss of O-linked glycosylation and increased receptor dimerization. J Biol Chem 289:5820-7 (2014). WB ; Human . Read more (PubMed: 24403076) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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