Recombinant
RabMAb

Anti-GCSF Receptor antibody [EPR8957] (ab156878)

Overview

  • Product name
    Anti-GCSF Receptor antibody [EPR8957]
    See all GCSF Receptor primary antibodies
  • Description
    Rabbit monoclonal [EPR8957] to GCSF Receptor
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) to residues in the intracellular domain of Human GCSF Receptor. (Q99062).

  • Positive control
    • Human placenta extract, NCCIT cell lysate, Caco-2 cell lysate
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab156878 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 92 kDa.
Flow Cyt 1/500 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Function
      Receptor for granulocyte colony-stimulating factor (CSF3), essential for granulocytic maturation. Plays a crucial role in the proliferation, differientation and survival of cells along the neutrophilic lineage. In addition it may function in some adhesion or recognition events at the cell surface.
    • Tissue specificity
      One or several isoforms have been found in myelogenous leukemia cell line KG-1, leukemia U-937 cell line, in bone marrow cells, placenta, and peripheral blood granulocytes. Isoform GCSFR-2 is found only in leukemia U-937 cells. Isoform GCSFR-3 is highly expressed in placenta.
    • Involvement in disease
      Hereditary neutrophilia
    • Sequence similarities
      Belongs to the type I cytokine receptor family. Type 2 subfamily.
      Contains 5 fibronectin type-III domains.
      Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    • Domain
      The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
      The box 1 motif is required for JAK interaction and/or activation.
    • Cellular localization
      Secreted and Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD 114 antibody
      • CD114 antibody
      • CD114 antigen antibody
      • Colony stimulating factor 3 receptor (granulocyte) antibody
      • Colony stimulating factor 3 receptor antibody
      • CSF 3R antibody
      • CSF3R antibody
      • CSF3R_HUMAN antibody
      • Csfgr antibody
      • G CSF R antibody
      • G-CSF receptor antibody
      • G-CSF-R antibody
      • GCSFR antibody
      • Granulocyte colony stimulating factor receptor antibody
      • Granulocyte colony-stimulating factor receptor antibody
      • OTTHUMP00000009703 antibody
      • OTTHUMP00000009704 antibody
      • OTTHUMP00000009705 antibody
      see all

    Images

    • Flow cytometric analysis of permeabilized NCCIT cells labeling GCSF Receptor using ab156878 antibody at a dilution of 1/500 (red) or a rabbit IgG (negative) (green).
    • All lanes : Anti-GCSF Receptor antibody [EPR8957] (ab156878) at 1/1000 dilution

      Lane 1 : Human placenta extract
      Lane 2 : NCCIT cell lysate
      Lane 3 : Caco-2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Developed using the ECL technique.

      Predicted band size: 92 kDa

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:
    • Wu Y  et al. Expression and role of granulocyte macrophage colony-stimulating factor receptor (GM-CSFR) and granulocyte colony-stimulating factor receptor (G-CSFR) on Ph-positive acute B lymphoblastic leukemia. Hematology N/A:1-9 (2018). Human . Read more (PubMed: 29338593) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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