Anti-GCSF Receptor antibody [S-1268] (ab18627)


  • Product nameAnti-GCSF Receptor antibody [S-1268]
    See all GCSF Receptor primary antibodies
  • Description
    Mouse monoclonal [S-1268] to GCSF Receptor
  • SpecificityDoes not cross-react with MBP or BSA.
  • Tested applicationsSuitable for: ELISA, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant MBP-GCSFR fusion protein (Human).



Our Abpromise guarantee covers the use of ab18627 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Predicted molecular weight: 92 kDa.
ICC/IF 1/500. See Abreview.


  • FunctionReceptor for granulocyte colony-stimulating factor (CSF3), essential for granulocytic maturation. Plays a crucial role in the proliferation, differientation and survival of cells along the neutrophilic lineage. In addition it may function in some adhesion or recognition events at the cell surface.
  • Tissue specificityOne or several isoforms have been found in myelogenous leukemia cell line KG-1, leukemia U-937 cell line, in bone marrow cells, placenta, and peripheral blood granulocytes. Isoform GCSFR-2 is found only in leukemia U-937 cells. Isoform GCSFR-3 is highly expressed in placenta.
  • Involvement in diseaseHereditary neutrophilia
  • Sequence similaritiesBelongs to the type I cytokine receptor family. Type 2 subfamily.
    Contains 5 fibronectin type-III domains.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
  • DomainThe WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    The box 1 motif is required for JAK interaction and/or activation.
  • Cellular localizationSecreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 114 antibody
    • CD114 antibody
    • CD114 antigen antibody
    • Colony stimulating factor 3 receptor (granulocyte) antibody
    • Colony stimulating factor 3 receptor antibody
    • CSF 3R antibody
    • CSF3R antibody
    • CSF3R_HUMAN antibody
    • Csfgr antibody
    • G CSF R antibody
    • G-CSF receptor antibody
    • G-CSF-R antibody
    • GCSFR antibody
    • Granulocyte colony stimulating factor receptor antibody
    • Granulocyte colony-stimulating factor receptor antibody
    • OTTHUMP00000009703 antibody
    • OTTHUMP00000009704 antibody
    • OTTHUMP00000009705 antibody
    see all

Anti-GCSF Receptor antibody [S-1268] images

  • ab18627 staining human Bone Marrow cells by ICC/IF.  Cells were PFA fixed and permeabilized in 1% Triton X-100 prior to blocking in 0.5% BSA for 1 hour at 25°C.  The primary antibody was diluted 1/500 and incubated with the sample for 2 hours at 25°C.  A FITC conjugated goat anti-mouse antibody, diluted 1/100, was used as the secondary.  The image on the left shows the 40X view of Bone Marrow cells stained for GCSFR showing the FITC stain predominantly on cell membranes.  The righthand image is the same image with DAPI overlay to show the cell nuclei.

    See Abreview

References for Anti-GCSF Receptor antibody [S-1268] (ab18627)

ab18627 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Bone Marrow)
Specification Bone Marrow
Fixative Paraformaldehyde
Permeabilization Yes - 1% Triton X-100
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.5% · Temperature: 25°C

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Submitted May 05 2008