Overview

  • Product name
  • Description
    Rabbit polyclonal to GDF 9
  • Specificity
    This antibody is specific for GDF 9 (N term).
  • Tested applications
    Suitable for: ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A KLH conjugated synthetic peptide (10-30 aa in length) at the N-term of first 60 aa of human GDF 9.

  • Positive control
    • HL60 cell lysate and hepatocarcinoma tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    This antibody is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab38544 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
IHC-P 1/50 - 1/100.
WB 1/100 - 1/500. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).

Target

  • Relevance
    GDF 9 is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Growth factors synthesized by ovarian somatic cells directly affect oocyte growth and function. GDF 9 is expressed in oocytes and is thought to be required for ovarian folliculogenesis.
  • Cellular localization
    Secreted
  • Database links
  • Alternative names
    • GDF9 antibody
    • Growth differentiation factor 9 antibody

Anti-GDF 9 antibody images

  • Anti-GDF 9 antibody (ab38544) at 1/100 dilution + HL60 cell lysate

    Predicted band size : 51 kDa
    Observed band size : 51 kDa
  • Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ab38544 at a 1/50 dilution, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.

    This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

References for Anti-GDF 9 antibody (ab38544)

This product has been referenced in:
  • Hu X  et al. Stem cells derived from human first-trimester umbilical cord have the potential to differentiate into oocyte-like cells in vitro. Int J Mol Med 35:1219-29 (2015). Read more (PubMed: 25760093) »
  • Wang Y  et al. Extra-ovarian expression and activity of growth differentiation factor 9. J Endocrinol 202:419-30 (2009). Read more (PubMed: 19505950) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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