Anti-GDF 9 antibody (ab93892)

Overview

  • Product nameAnti-GDF 9 antibody
    See all GDF 9 primary antibodies
  • Description
    Rabbit polyclonal to GDF 9
  • Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Rat, Sheep, Human, Pig, Marmoset (common)
    Predicted to work with: Mouse, Rabbit, Goat, Chicken, Cat, Dog, Baboon
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus of Human GDF 9.

  • Positive control
    • In Western Blot, this antibody gave a positive signal in Human Ovary Tissue Lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab93892 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).

Target

  • RelevanceGDF 9 is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Growth factors synthesized by ovarian somatic cells directly affect oocyte growth and function. GDF 9 is expressed in oocytes and is thought to be required for ovarian folliculogenesis.
  • Cellular localizationSecreted
  • Database links
  • Alternative names
    • GDF9 antibody
    • Growth differentiation factor 9 antibody

Anti-GDF 9 antibody images

  • ab93892 staining GDF 9 in Rat ovary tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 1.5% serum for 1 hour at 24°C; antigen retrieval was enzymatic. Samples were incubated with primary antibody (1μg/ml) for 12 hours at 4°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • Anti-GDF 9 antibody (ab93892) at 1 µg/ml + Human ovary tissue lysate - total protein (ab30222) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 51 kDa
    Observed band size : 51 kDa


    Exposure time : 2 minutes
  • IHC image of GDF 9 staining in human normal testis FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab93892, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
  • ICC/IF image of ab93892 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab93892 at 5µg/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemical analysis of oocytes of primordial (A), and early antral follicles (B) of sheep ovary tissue, staining GDF 9 with ab93892.

    Tissue was fixed with paraformaldehyde and blocked with 0.4% blocking agent for 5 minutes at 20°C; antigen retrieval was by heat mediation in citrate buffer (pH 6). Samples were incubated with primary antibody (1/1500 in diluent) for 24 hours at 4°C. An undiluted HRP-conjugated goat anti-rabbit polyclonal IgG was used as the secondary antibody.

    See Abreview

References for Anti-GDF 9 antibody (ab93892)

ab93892 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer
Sample Baboon Tissue sections (Ovary)
Specification Ovary
Permeabilization Yes - TBS with 0.1% Tween 20
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Sep 26 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako antigen retrieval solution
Sample Marmoset (common) Tissue sections (Adult ovary)
Specification Adult ovary
Permeabilization No
Fixative Formaldehyde
Username

煜欽 林

Verified customer

Submitted Oct 31 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 45 minute(s) · Concentration: 1.5% · Temperature: 25°C
Antigen retrieval step Enzymatic
Sample Rat Tissue sections (GONAD)
Specification GONAD
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Sep 23 2013

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: 24°C
Antigen retrieval step Enzymatic
Sample Rat Tissue sections (Ovary)
Specification Ovary
Permeabilization No
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jul 16 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Sheep Tissue sections (Ovary)
Specification Ovary
Fixative Paraformaldehyde
Antigen retrieval step Other
Permeabilization No
Blocking step Casein (Protein Block, Novocastra Ref. RE7102) as blocking agent for 5 minute(s) · Concentration: 0.4% · Temperature: 20°C
Username

Mrs. Rosa Ana Picazo

Verified customer

Submitted Oct 19 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Pig Tissue sections (ovary)
Specification ovary
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Dr. michal Shachar

Verified customer

Submitted Apr 19 2012

Thank you for your enquiry. So I can complete the information regarding your testing discount promotion code, please let me know which institution you are working at. Thanks for the additional information!

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"