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A synthetic peptide corresponding to a region near the amino terminal end of Human GDF15 protein. A residue of cysteine was added to facilitate coupling to KLH.
Our Abpromise guarantee covers the use of ab106112 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 34 kDa.|
ICC/IF image of GDF15 staining on human blood film using ab106112 (1:1000). The cells were fixed using formaldehyde and permeabilized using 0.1% TritonX in 0.1% PBS. The cells were then blocked using 10% donkey serum for 1 hour at 24°C. ab106112 was diluted to 1:1000 and incubated with cells for 4 hours at 24°C. The secondary antibody used was donkey polyclonal to mouse IgG conjugated to Alexa Fluor 568.
ab106112 has not yet been referenced specifically in any publications.