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Our Abpromise guarantee covers the use of ab39999 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 34 kDa).|
|IHC-Fr||1/200. See Abreview.|
|IHC-P||Use a concentration of 5 µg/ml.|
|Flow Cyt||Use a concentration of 10 µg/ml.|
Paraffin-embedded human placenta tissue stained for GDF-15 using ab39999 at 5 μg/ml in immunohistochemical analysis. Steamed antigen retrieval with citrate buffer pH 6.
ICC/IF image of human bone marrow smear stained with Ab39999(1/250). The smear was permeabilized in 0.1% PBS-Triton X for 1h and incubated with 10% donkey serum for 1hr at 24°C to block non-specific protein-protein interactions. The sections were then incubated with ab39999 used at 1/250 dilution, overnight at +4°C. The secondary antibody was Alexa Fluor® 568 donkey anti-goat used at a 1/1000 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Paraffin-embedded human prostate tissue stained for GDF-15 using ab39999 at 5 μg/ml in immunohistochemical analysis. Steamed antigen retrieval with citrate buffer pH 6.
Flow cytometric analysis of paraformaldehyde fixed, 0.5% Triton permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling GDF-15 using ab39999 at 10 μg/ml followed by Alexa Fluor 488 secondary antibody (blue) at 1 μg/ml. Unimmunized goat IgG was used as isotype control, followed by Alexa Fluor 288 secondary antibody (black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"