Overview

  • Product name
  • Description
    Goat polyclonal to GDF15
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-Frmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide: QKTDTGVSLQTYDD, corresponding to C terminal amino acids 286-299 of Human GDF15

  • Positive control
    • Human prostate lysate. Lysate prepared from transfected HEK293 cells transiently expressing GDF15.

Properties

Applications

Our Abpromise guarantee covers the use of ab39999 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 34 kDa).
ICC/IF 1/250.
IHC-Fr 1/200. See Abreview.

Target

  • Tissue specificity
    Highly expressed in placenta, with lower levels in prostate and colon and some expression in kidney.
  • Sequence similarities
    Belongs to the TGF-beta family.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • GDF 15 antibody
    • GDF-15 antibody
    • Gdf15 antibody
    • GDF15_HUMAN antibody
    • Growth differentiation factor 15 antibody
    • Growth/differentiation factor 15 antibody
    • Macrophage inhibitory cytokine 1 antibody
    • MIC 1 antibody
    • Mic-1 antibody
    • MIC1 antibody
    • NAG 1 antibody
    • NAG-1 antibody
    • NAG1 antibody
    • NRG 1 antibody
    • NRG-1 antibody
    • NRG1 antibody
    • NSAID (nonsteroidal anti inflammatory drug) activated protein 1 antibody
    • NSAID antibody
    • NSAID regulated protein 1 antibody
    • NSAID-activated gene 1 protein antibody
    • NSAID-regulated gene 1 protein antibody
    • PDF antibody
    • PLAB antibody
    • Placental bone morphogenetic protein antibody
    • Placental bone morphogenic protein antibody
    • Placental TGF beta antibody
    • Placental TGF-beta antibody
    • Prostate differentiation factor antibody
    • PTGF beta antibody
    • PTGFB antibody
    see all

Images

  • ICC/IF image of human bone marrow smear stained with Ab39999(1/250). The smear was permeabilized in 0.1% PBS-Triton X for 1h and incubated with 10% donkey serum for 1hr at 24°C to block non-specific protein-protein interactions. The sections were then incubated with ab39999 used at 1/250 dilution, overnight at +4°C. The secondary antibody was Alexa Fluor® 568 donkey anti-goat used at a 1/1000 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    See Abreview

  • Anti-GDF15 antibody (ab39999) at 0.3 µg/ml + Human Prostate lysate in RIPA buffer at 35 µg

    Predicted band size : 34 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)
    Primary incubation was 1 hour. Detected by chemiluminescence.
  • Anti-GDF15 antibody (ab39999) at 0.3 µg/ml + lysate prepared from transfected HEK293 cells transiently expressing GDF15

    Predicted band size : 34 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)

References

This product has been referenced in:
  • Gabriel KN  et al. Association and regulation of protein factors of field effect in prostate tissues. Int J Oncol 49:1541-1552 (2016). ICC/IF ; Human . Read more (PubMed: 27634112) »
  • Zhou YM  et al. Growth differentiation factor-15 (GDF-15), novel biomarker for assessing atrial fibrosis in patients with atrial fibrillation and rheumatic heart disease. Int J Clin Exp Med 8:21201-7 (2015). Read more (PubMed: 26885055) »

See all 5 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (human colon)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mm sodium citrate
Permeabilization
No
Specification
human colon
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Oct 16 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Adipose and MEFs)
Gel Running Conditions
Reduced Denaturing (8%)
Loading amount
25 µg
Treatment
Epididymal Fast/Refed and MEFs with Tunicamycin (6h)
Specification
Adipose and MEFs
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Satish Patel

Verified customer

Submitted Aug 01 2017

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (15% gel)
Sample
Human Cell lysate - whole cell (HELA)
Specification
HELA
Treatment
10uM Tunicamycin for 24hrs
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Oct 30 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Sample
Human Cell (Bone Marrow)
Specification
Bone Marrow
Permeabilization
Yes - 0.1% TritonX in 0.1% PBS
Fixative
Paraformaldehyde
Username

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Aug 06 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (aortic plaque)
Specification
aortic plaque
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 20°C
Username

Miss. Silke Vorwald

Verified customer

Submitted Jun 04 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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