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Rabbit polyclonal to GDF15
Synthetic peptide conjugated to KLH derived from within residues 250 to the C-terminus of Human GDF15.
(Peptide available as
This antibody gave a positive signal in the following human tissue lysates: Prostate; Colon.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 µg/ml.
Use a concentration of 1 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
Highly expressed in placenta, with lower levels in prostate and colon and some expression in kidney.
Belongs to the TGF-beta family.
Information by UniProt
GDF 15 antibody
Western blot - GDF15 antibody (ab82569)
All lanes :
Anti-GDF15 antibody (ab82569) at 1 µg/ml
Lane 1 :
Prostate (Human) Cytoplasmic Lysate - adult normal tissue (
Lane 2 :
Human colon tissue lysate - total protein (
Lysates/proteins at 10 µg/ml per lane.
Secondary All lanes :
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size:
Observed band size:
Additional bands at:
53 kDa. We are unsure as to the identity of these extra bands.
Immunocytochemistry/ Immunofluorescence - GDF15 antibody (ab82569)
ICC/IF image of ab82569 stained HeLa cells. The cells were 100% Methanol fixed (5 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab82569, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"