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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
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Ammonium Sulphate Precipitation
ab71250 is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
1/10 - 1/50.
Anti-GDF3 antibody images
Western blot - GDF3 antibody (ab71250)
Anti-GDF3 antibody (ab71250) at 1/60 dilution + HepG2 cell line lysates at 35 µg
Predicted band size : 41 kDa Observed band size : 41 kDa Additional bands at : 25 kDa,60 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-GDF3 antibody(ab71250)
ab71250 at 1/50 dilution staining GDF3 in human testis tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). A peroxidase conjugated secondary antibody was used followed by DAB staining.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"