Recombinant Anti-GDF7 antibody [EPR16000] (ab189928)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16000] to GDF7
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-GDF7 antibody [EPR16000]
See all GDF7 primary antibodies -
Description
Rabbit monoclonal [EPR16000] to GDF7 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal brain, C6 and Neuro-2a cell lysates; Human adenocarcinoma of colon and rat brain tissues, U87-MG cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16000 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab189928 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 52 kDa (predicted molecular weight: 47 kDa).
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IHC-P |
1/1000 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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ICC/IF |
1/50.
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 52 kDa (predicted molecular weight: 47 kDa). |
IHC-P
1/1000 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
1/50. |
Target
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Function
May play an active role in the motor area of the primate neocortex. -
Sequence similarities
Belongs to the TGF-beta family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 151449 Human
- Entrez Gene: 238057 Mouse
- Entrez Gene: 252833 Rat
- Omim: 604651 Human
- SwissProt: Q7Z4P5 Human
- SwissProt: P43029 Mouse
- Unigene: 447688 Human
- Unigene: 271308 Mouse
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Alternative names
- bmp12 antibody
- bone morphogenetic protein 12 antibody
- GDF-7 antibody
see all
Images
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Immunocytochemical analysis of 4% paraformaldehyde fixed U87-MG cells labeling GDF7 with ab189928 at 1/50 dilution, and Goat anti rabbit IgG(Alexa Fluor® 555) at 1/200 as a secondary antibody. Counterstained with DAPI.
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Anti-Spa-1 antibody [EPR14134] (ab189929) at 1/10000 dilution + Human fetal brain tissue lysate at 20 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 47 kDa
Additional bands at: 52 kDa. We are unsure as to the identity of these extra bands. -
All lanes : Anti-GDF7 antibody [EPR16000] (ab189928) at 1/1000 dilution
Lane 1 : C6 cell lysate
Lane 2 : Neuro-2a cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 47 kDa -
Immunohistochemical analysis of formalin fixed, paraffin embedded Human adenocarcinoma of colon tissue labeling GDF7 with ab189928 at 1/2000 dilution, and prediluted HRP Polymer for Rabbit IgG as a secondary antibody. Counterstained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of formalin fixed, paraffin embedded Human adenocarcinoma of colon labeling GDF7 with ab189928 at 1/2000 dilution, and prediluted HRP Polymer for Rabbit IgG as a secondary antibody. Counterstained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab189928 has been referenced in 1 publication.
- Fu W et al. Mesenchymal Behavior of the Endothelium Promoted by SMAD6 Downregulation Is Associated With Brain Arteriovenous Malformation Microhemorrhage. Stroke 51:2197-2207 (2020). PubMed: 32486965