Overview

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab98337 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).

Target

Anti-GDF8 / Myostatin antibody images

  • All lanes : Anti-GDF8 / Myostatin antibody (ab98337) at 1/250 dilution

    Lane 1 : Human skeletal muscle tissue lysate - total protein (ab29330)
    Lane 2 : Skeletal Muscle (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 43 kDa
    Observed band size : 43 kDa
    Additional bands at : 28 kDa (possible cleavage fragment).

    Exposure time : 16 minutes
  • All lanes : Anti-GDF8 / Myostatin antibody (ab98337) at 1/250 dilution

    Lane 1 : Recombinant human GDF8 / Myostatin protein (ab73251) at 0.1 µg
    Lane 2 : Recombinant human GDF8 / Myostatin protein (ab73251) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 43 kDa


    Exposure time : 30 seconds

    ab73251 is a homodimer consisting of two 109aa monomers. The homodimer expected size is 25kDa, so the observed band is thought to be the monomer form.

References for Anti-GDF8 / Myostatin antibody (ab98337)

This product has been referenced in:
  • Yu B  et al. Efficient TALEN-mediated myostatin gene editing in goats. BMC Dev Biol 16:26 (2016). WB ; Goat . Read more (PubMed: 27461387) »
  • Ma D  et al. Over-Expression of Porcine Myostatin Missense Mutant Leads to A Gender Difference in Skeletal Muscle Growth between Transgenic Male and Female Mice. Int J Mol Sci 16:20020-32 (2015). Read more (PubMed: 26305245) »

See all 7 Publications for this product

Product Wall

Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Non-Denaturing (Native)
Sample
Human Cell lysate - whole cell (Gastrocnemius)
Specification
Gastrocnemius
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C
Username

Dr. Francesco Elia Marino

Verified customer

Submitted Mar 12 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10 % gel)
Sample
Chicken Tissue lysate - whole (skeletal muscle)
Specification
skeletal muscle
Blocking step
BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Jul 17 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Horse Tissue lysate - whole (skeletal muscle)
Loading amount
45 µg
Specification
skeletal muscle
Gel Running Conditions
Reduced Denaturing (4%stacking, 10% running)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Pippa Morrison

Verified customer

Submitted Nov 12 2012

That is no problem. If you have any further questions, please do not hesitate to contact us.


As you are interested in participating in the testing discount scheme, I have generated a discount code for you to use (See below). Once you ha...

Read More

Thank you for contacting us.


I have performed a sequence comparison of the peptide immunogen used to raise this antibody to that of equine myostatin (SwissProt reference http://www.uniprot.org/uniprot/Q9GM97). The sequences match 100%, ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up