The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
FunctionRegulates the GDP/GTP exchange reaction of most Rab proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them.
Tissue specificityBrain; predominant in neural and sensory tissues.
Involvement in diseaseDefects in GDI1 are the cause of mental retardation X-linked type 41 (MRX41) [MIM:300104]. Mental retardation is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Non-syndromic mental retardation patients do not manifest other clinical signs. Defects in GDI1 are the cause of mental retardation X-linked type 48 (MRX48) [MIM:300104]; also known as MRX3.
Sequence similaritiesBelongs to the Rab GDI family.
Western blot analysis of HeLa (lane 1) NIHT3 (lane 2) and IHKE1 (lane 3) cell lysates (40µg/lane) labeling GDI1 with ab23354 at 1:1000. A HRP-conjugated Goat anti-rabbit IgG (1:1000) was used as the secondary antibody.
Western blot - GDI1 antibody (ab23354)
Anti-GDI1 antibody (ab23354) at 1.25 µg/ml + HepG2 whole cell lysate with 5% skimmed milk in PBS
Secondary HRP conjugated anti-Rabbit IgG at 1/50000 dilution