Overview

  • Product nameAnti-GDI2 antibody
    See all GDI2 primary antibodies
  • Description
    Rabbit polyclonal to GDI2
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Cow, Dog, Pig, Caenorhabditis elegans
  • Immunogen

    A region within synthetic peptide: EPEKEIRPAL ELLEPIEQKF VSISDLLVPK DLGTESQIFI SRTYDATTHF, corresponding to amino acids 361-410 of Human GDI2

  • Positive control
    • HepG2 cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab49193 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 51 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Anti-GDI2 antibody images

  • All lanes : Anti-GDI2 antibody (ab49193) at 0.5 µg/ml

    Lane 1 : Molecular weight marker
    Lane 2 : HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution
    Developed using the ECL technique

    Predicted band size : 51 kDa
    Observed band size : 48 kDa (why is the actual band size different from the predicted?)

References for Anti-GDI2 antibody (ab49193)

This product has been referenced in:
  • Hibbert B  et al. Glycogen synthase kinase-3ß inhibition augments diabetic endothelial progenitor cell abundance and functionality via cathepsin B: a novel therapeutic opportunity for arterial repair. Diabetes 63:1410-21 (2014). WB ; Human . Read more (PubMed: 24296714) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (astrocytroma)
Loading amount 25 µg
Specification astrocytroma
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
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Verified customer

Submitted Apr 10 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"