• Product nameAnti-GDNF antibody
    See all GDNF primary antibodies
  • Description
    Rabbit polyclonal to GDNF
  • SpecificityGDNF homodimerizes and has 5 isoforms along with a signal and propeptide, so there is a potential for multiple bands in western blot.
  • Tested applicationsSuitable for: IHC-P, IHC-Fr, ICC/IF, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide surrounding amino acid 195 of mouse GDNF.(Peptide available as ab51926.)



Our Abpromise guarantee covers the use of ab18956 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
IHC-Fr Use a concentration of 10 - 20 µg/ml.
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 0.5 - 4 µg/ml. Predicted molecular weight: 24 kDa.Can be blocked with GDNF peptide (ab51926).

Western blot may give multiple bands. GDNF homodimerizes and has 5 isoforms along with a signal and propeptide.

IP Use a concentration of 10 - 20 µg/ml.


  • FunctionNeurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.
  • Tissue specificityIn the brain, predominantly expressed in the striatum with highest levels in the caudate and lowest in the putamen.
  • Involvement in diseaseDefects in GDNF may be a cause of Hirschsprung disease (HSCR) [MIM:142623]. In association with mutations of RET gene, defects in GDNF may be involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction.
    Defects in GDNF are a cause of congenital central hypoventilation syndrome (CCHS) [MIM:209880]; also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia.
  • Sequence similaritiesBelongs to the TGF-beta family. GDNF subfamily.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Astrocyte derived trophic factor antibody
    • Astrocyte derived trophic factor 1 antibody
    • Astrocyte-derived trophic factor antibody
    • ATF 1 antibody
    • ATF 2 antibody
    • Atf antibody
    • ATF1 antibody
    • ATF2 antibody
    • gdnf antibody
    • GDNF_HUMAN antibody
    • Glial cell derived neurotrophic factor antibody
    • Glial Cell Line Derived Neurotrophic Factor antibody
    • Glial cell line-derived neurotrophic factor antibody
    • Glial derived neurotrophic factor antibody
    • HFB1 GDNF antibody
    • hGDNF antibody
    • HSCR3 antibody
    see all

Anti-GDNF antibody images

  • ab18956 staining GDNF in rat brain tissue sections by IHC-P (Formalin/PFA-fixed paraffin-embedded sections). Cells were fixed with paraformaledhyde, permeabilized with 0.1% Tween-20 and PBS and blocked with 1% BSA for 30 minutes at 25°C; antigen retrieval was by heat mediation in a citrate buffer pH 6.0. Samples were incubated with primary antibody (1/150 in TBS) for 24 hours at 4oC. ab96883 at a dilution of 1/200 was used as the secondary antibody.

    Green = GDNF, Blue = DAPI stained nuclei.

  • ICC/IF image of ab18956 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab18956, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of ab18956 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab18956, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-GDNF antibody (ab18956)

This product has been referenced in:
  • Quan FS  et al. Comparative effect of immature neuronal or glial cell transplantation on motor functional recovery following experimental traumatic brain injury in rats. Exp Ther Med 12:1671-1680 (2016). IF . Read more (PubMed: 27602084) »
  • Moghimi E  et al. Dietary Vitamin D3 Restriction Exacerbates Disease Pathophysiology in the Spinal Cord of the G93A Mouse Model of Amyotrophic Lateral Sclerosis. PLoS One 10:e0126355 (2015). WB ; Mouse . Read more (PubMed: 26020962) »

See all 11 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Cell (Rat Tissue sections -Brain)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer pH 6.0
Permeabilization Yes - 0.1% Tween 20 in PBS
Specification Rat Tissue sections -Brain
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative Paraformaldehyde

Ms. Chia-Li Lin

Verified customer

Submitted Nov 20 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Dako Flex peroxidase as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: RT°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: High pH
Sample Human Tissue sections (Tendon)
Specification Tendon
Permeabilization No
Fixative 10% buffered formalin

Abcam user community

Verified customer

Submitted Oct 20 2014

Thank you for contacting us.

The detection limit of this antibody in WB has not been tested, but the antibody was validated using blocking peptide ab51926. The peptide was incubated with an equal volume of antibody for 30 minutes at 37C prior...

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Thank you for your call today and for letting us know about the trouble with the new lot of ab18956.

I am sorry that the new lot of this antibody also did not work as expected. I'm sending a vial of ab64337 free of charge on the order ***, wh...

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Thank you for your call today and for letting us know about the trouble with ab18956.

As we discussed, I'm sending a free of charge vial from a different lot on the order ***, which should arriveTuesday or Wednesday ofnext week.


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Thank you for contacting Abcam.

For ab18956 and ab6199, heat mediated antigen retrieval would work well for both. As for which serum to use, that will depend on the host species of your secondary antibody. If the secondary antibody is used in...

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Thank you for contacting Abcam.

I have attached our protocols book that contains a general IHC-P protocol that is a good starting point.

Also for more specific protocols related to these antibodies, there a links below to Abreviews ...

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Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody. The details you have kindly provided will enable us to investigate this ca...

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Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Midbrain sections--transduced with rAAV-hGDNF)
Specification Midbrain sections--transduced with rAAV-hGDNF
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization Yes - 0.1% triton X-100
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Apr 01 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (293T)
Loading amount 4 µg
Specification 293T
Treatment Transfected with human GDNF cDNA
Gel Running Conditions Reduced Denaturing (12)
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Feb 18 2008