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Synthetic peptide surrounding amino acid 195 of mouse GDNF.(Peptide available as ab51926.)
Our Abpromise guarantee covers the use of ab18956 in the following tested applications.
|IHC-P||Use a concentration of 5 µg/ml.|
|IHC-Fr||Use a concentration of 10 - 20 µg/ml.|
|ICC/IF||Use at an assay dependent concentration.|
|WB||Use a concentration of 0.5 - 4 µg/ml. Predicted molecular weight: 24 kDa.Can be blocked with GDNF peptide (ab51926).
Western blot may give multiple bands. GDNF homodimerizes and has 5 isoforms along with a signal and propeptide.
|IP||Use a concentration of 10 - 20 µg/ml.|
Immunocytochemistry/ Immunofluorescence analysis of genetically modified rat embryonic fibroblasts labeling GDNF with ab18956 at 1/150 dilution. Cells were fixed in formaldehyde and permeabilized with Tx-100. Staining with ab18956 at 1/150 was carried out for 14 hours at 4°C in PBS buffer. A Goat Anti-Rabbit (Alexa Fluor® 594) secondary antibody was used at 1/600 dilution.
ab18956 staining GDNF in rat brain tissue sections by IHC-P (Formalin/PFA-fixed paraffin-embedded sections). Cells were fixed with paraformaledhyde, permeabilized with 0.1% Tween-20 and PBS and blocked with 1% BSA for 30 minutes at 25°C; antigen retrieval was by heat mediation in a citrate buffer pH 6.0. Samples were incubated with primary antibody (1/150 in TBS) for 24 hours at 4oC. ab96883 at a dilution of 1/200 was used as the secondary antibody.
Green = GDNF, Blue = DAPI stained nuclei.