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Synthetic peptide surrounding amino acid 195 of mouse GDNF.(Peptide available as ab51926.)
Our Abpromise guarantee covers the use of ab18956 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml.|
|IHC-Fr||Use a concentration of 10 - 20 µg/ml.|
|ICC/IF||Use at an assay dependent concentration.|
|WB||Use a concentration of 0.5 - 4 µg/ml. Predicted molecular weight: 24 kDa.Can be blocked with GDNF peptide (ab51926).
Western blot may give multiple bands. GDNF homodimerizes and has 5 isoforms along with a signal and propeptide.
|IP||Use a concentration of 10 - 20 µg/ml.|
ab18956 staining GDNF in rat brain tissue sections by IHC-P (Formalin/PFA-fixed paraffin-embedded sections). Cells were fixed with paraformaledhyde, permeabilized with 0.1% Tween-20 and PBS and blocked with 1% BSA for 30 minutes at 25°C; antigen retrieval was by heat mediation in a citrate buffer pH 6.0. Samples were incubated with primary antibody (1/150 in TBS) for 24 hours at 4oC. ab96883 at a dilution of 1/200 was used as the secondary antibody.
Green = GDNF, Blue = DAPI stained nuclei.
Immunocytochemistry/ Immunofluorescence analysis of genetically modified rat embryonic fibroblasts labeling GDNF with ab18956 at 1/150 dilution. Cells were fixed in formaldehyde and permeabilized with Tx-100. Staining with ab18956 at 1/150 was carried out for 14 hours at 4°C in PBS buffer. A Goat Anti-Rabbit (Alexa Fluor® 594) secondary antibody was used at 1/600 dilution.
This image is courtesy of an anonymous abreview.