This antibody does not crossreact with TGF alpha, TGF beta 1, NGF or BDNF at up to 10 µg/ml. GDNF homodimerizes and has 5 isoforms along with a signal and propeptide, so there is a potential for multiple bands in western blot.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at a concentration of 1 µg/ml.
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 24 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.
In the brain, predominantly expressed in the striatum with highest levels in the caudate and lowest in the putamen.
Involvement in disease
Defects in GDNF may be a cause of Hirschsprung disease (HSCR) [MIM:142623]. In association with mutations of RET gene, defects in GDNF may be involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction. Defects in GDNF are a cause of congenital central hypoventilation syndrome (CCHS) [MIM:209880]; also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia.