Anti-GDNF antibody (ab28956)
Key features and details
- Chicken polyclonal to GDNF
- Suitable for: WB
- Reacts with: Rat, Human
- Isotype: IgY
Overview
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Product name
Anti-GDNF antibody
See all GDNF primary antibodies -
Description
Chicken polyclonal to GDNF -
Host species
Chicken -
Specificity
GDNF homodimerizes and has 5 isoforms along with a signal and propeptide, so there is a potential for multiple bands in western blot. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
This product was produced with the following immunogens:
Synthetic peptide corresponding to Human GDNF aa 1-100.
Synthetic peptide corresponding to Human GDNF aa 100-200. -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
IgY fraction -
Purification notes
Crude IgY preparation from egg yolks using a precipitation method (PEG, Polyethylene Glycol). -
Clonality
Polyclonal -
Isotype
IgY -
Research areas
Associated products
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab28956 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/2000. Predicted molecular weight: 24 kDa.
GDNF homodimerizes and has 5 isoforms along with a signal and propeptide, so there is a potential for multiple bands in western blot. |
Notes |
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WB
1/500 - 1/2000. Predicted molecular weight: 24 kDa. GDNF homodimerizes and has 5 isoforms along with a signal and propeptide, so there is a potential for multiple bands in western blot. |
Target
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Function
Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. -
Tissue specificity
In the brain, predominantly expressed in the striatum with highest levels in the caudate and lowest in the putamen. -
Involvement in disease
Defects in GDNF may be a cause of Hirschsprung disease (HSCR) [MIM:142623]. In association with mutations of RET gene, defects in GDNF may be involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction.
Defects in GDNF are a cause of congenital central hypoventilation syndrome (CCHS) [MIM:209880]; also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. -
Sequence similarities
Belongs to the TGF-beta family. GDNF subfamily. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 2668 Human
- Entrez Gene: 25453 Rat
- Omim: 600837 Human
- SwissProt: P39905 Human
- SwissProt: Q07731 Rat
- Unigene: 248114 Human
- Unigene: 53970 Rat
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Alternative names
- Astrocyte derived trophic factor antibody
- Astrocyte derived trophic factor 1 antibody
- Astrocyte-derived trophic factor antibody
see all
Images
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Anti-GDNF antibody (ab28956) + Rat brain total protein extract. at 40 µg
Secondary
Rabbit Anti-Chicken IgY (H&L) at 1/5000 dilution
Predicted band size: 24 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted?
Datasheets and documents
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Datasheet download
References (1)
ab28956 has been referenced in 1 publication.
- Yuan ZL et al. Activation of GDNF-ERK-Runx1 signaling contributes to P2X3R gene transcription and bone cancer pain. iScience 25:104936 (2022). PubMed: 36072549