Anti-GDNF antibody (Biotin) (ab84276)


  • Product nameAnti-GDNF antibody (Biotin)
    See all GDNF primary antibodies
  • Description
    Rabbit polyclonal to GDNF (Biotin)
  • ConjugationBiotin
  • SpecificityGDNF homodimerizes and has 5 isoforms along with a signal and propeptide, so there is a potential for multiple bands in western blot.
  • Tested applicationsSuitable for: ELISA, Sandwich ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Highly pure (>98%) recombinant human GDNF



Our Abpromise guarantee covers the use of ab84276 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA
  • Application notesELISA: Use at a concentration of 0.25 - 1 µg/ml. Allows the detection of at least 0.2 - 0.4 ng/well of recombinant human GDNF.
    sELISA: Use at a concentration of 0.25 - 1 µg/ml. Allows the detection of at least 0.2 - 0.4 ng/well of recombinant human GDNF.
    WB: Use at a concentration of 0.1 - 0.2 µg/ml. The detection limit for recombinant human GDNF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Predicted molecular weight: 24 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionNeurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.
    • Tissue specificityIn the brain, predominantly expressed in the striatum with highest levels in the caudate and lowest in the putamen.
    • Involvement in diseaseDefects in GDNF may be a cause of Hirschsprung disease (HSCR) [MIM:142623]. In association with mutations of RET gene, defects in GDNF may be involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction.
      Defects in GDNF are a cause of congenital central hypoventilation syndrome (CCHS) [MIM:209880]; also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia.
    • Sequence similaritiesBelongs to the TGF-beta family. GDNF subfamily.
    • Cellular localizationSecreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • Astrocyte derived trophic factor antibody
      • Astrocyte derived trophic factor 1 antibody
      • Astrocyte-derived trophic factor antibody
      • ATF 1 antibody
      • ATF 2 antibody
      • Atf antibody
      • ATF1 antibody
      • ATF2 antibody
      • gdnf antibody
      • GDNF_HUMAN antibody
      • Glial cell derived neurotrophic factor antibody
      • Glial Cell Line Derived Neurotrophic Factor antibody
      • Glial cell line-derived neurotrophic factor antibody
      • Glial derived neurotrophic factor antibody
      • HFB1 GDNF antibody
      • hGDNF antibody
      • HSCR3 antibody
      see all

    References for Anti-GDNF antibody (Biotin) (ab84276)

    ab84276 has not yet been referenced specifically in any publications.

    Product Wall

    We do not have any data for non-reduced samples, and have not received other reports of a band at 60 kDa. I have looked at datasheets of other GDNF antibodies for evidence of a tetramer but none of them have been tested on non-reduced samples either. I...

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