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Products:Neuroscience >> Neurology process >> Growth and Development >> Neurotrophins
Overview
Product name
Anti-GDNF Receptor alpha 1 antibody
See all GDNF Receptor alpha 1 products (7) ...
Description
Rabbit polyclonal to GDNF Receptor alpha 1
Tested applications
IHC (PFA fixed), IHC-P, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide, corresponding to amino acids 369-382 of Human GFR alpha 1.
Positive control
Human brain lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
IgG fraction
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Neurology process >> Growth and Development >> Neurotrophins
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Western blot - GDNF Receptor alpha 1 antibody (ab8026)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab8026 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC (PFA fixed)
- 1 Image
WB
Application notes
IHC-P: Use at a concentration of 1 µg/ml. Perform heat mediated antigen retrieval (citrate buffer 10 mM, pH 6.0) before commencing with IHC staining protocol.
Immunohistochemistry (PFA fixed): 1/50.
WB: 1/500. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
Glial cell line-derived neurotrophic factor (GDNF) is a potent survival factor for central and peripheral neurons and is essential for the development of kidneys and the enteric nervous system. Physiological responses to GDNF require the presence of a novel glycosylphosphadidylinositol linked protein GDNFRa, which is a cell surface receptor for GDNF. The cDNAs encoding GDNFRa from human, rat, chicken and mouse have been cloned recently. GDNFRa was also termed Ret ligand 1 (RETL1) or TGF b related neurotrophic factor receptor 1 (TrnR1) and nominated as GFRa 1. GFRa 1 binds GDNF specifically and mediates activation of the Ret protein tyrosine kinase (PTK). Thus, GDNF, GFRa and the Ret PTK form a complex to transduce GDNF signal and to mediate GDNF function.
Cellular localization
Cell Membrane
Alternative names
- TRNR1 antibody
- GDNF Family Receptor Alpha 1 antibody
- GDNF R antibody
see all
Database links
- Entrez Gene: 2674 Human
- Entrez Gene: 14585 Mouse
- Entrez Gene: 25454 Rat
- Omim: 601496 Human
- SwissProt: P56159 Human
- SwissProt: O35246 Mouse
- SwissProt: O35252 Mouse
- SwissProt: P97785 Mouse
see all
Anti-GDNF Receptor alpha 1 antibody images:
Western blot - GDNF Receptor alpha 1 antibody (ab8026)
All lanes : Anti-GDNF Receptor alpha 1 antibody (ab8026) at 1/500 dilution
Lane 1 : Crude membrane fractions of human brain
Lane 2 : Crude membrane fractions of liver
Lane 3 : Crude membrane fractions of kidney
Lane 4 : Crude membrane fractions of spleen
Predicted band size : 50 kDa
Observed band size : 50 kDa
Protocols
References for Anti-GDNF Receptor alpha 1 antibody (ab8026)
This product has been referenced in:
- He Zet al. Isolation, characterization, and culture of human spermatogonia. Biol Reprod 82:363-72 (2010). IHC-P; Human.Read more (PubMed: 19846602) »
- Serra MPet al. Ret, GFRalpha-1, GFRalpha-2 and GFRalpha-3 receptors in the human hippocampus and fascia dentata. Int J Dev Neurosci 23:425-38 (2005).Read more (PubMed: 16002253) »
See all 2 publications for this product
Publishing research using ab8026? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"