Anti-GDNF Receptor alpha 1 antibody (ab8026)

Overview

  • Product name
    Anti-GDNF Receptor alpha 1 antibody
    See all GDNF Receptor alpha 1 primary antibodies
  • Description
    Rabbit polyclonal to GDNF Receptor alpha 1
  • Tested applications
    Suitable for: IHC (PFA fixed), IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to amino acids 369-382 of Human GFR alpha 1.

  • Positive control
    • Human brain lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    IgG fraction
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab8026 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC (PFA fixed)
IHC-P
WB
  • Application notes
    IHC-P: Use at a concentration of 1 µg/ml. Perform heat mediated antigen retrieval (citrate buffer 10 mM, pH 6.0) before commencing with IHC staining protocol.
    Immunohistochemistry (PFA fixed): 1/50.
    WB: 1/500. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Receptor for GDNF. Mediates the GDNF-induced autophosphorylation and activation of the RET receptor.
    • Sequence similarities
      Belongs to the GDNFR family.
    • Cellular localization
      Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • GDNF family receptor alpha 1 antibody
      • GDNF family receptor alpha-1 antibody
      • GDNF R antibody
      • GDNF RA antibody
      • GDNF receptor alpha antibody
      • GDNF receptor alpha-1 antibody
      • GDNFR alpha 1 antibody
      • GDNFR alpha antibody
      • GDNFR antibody
      • GDNFR-alpha-1 antibody
      • GDNFRA antibody
      • GDNFRalpha antibody
      • GFR alpha 1 antibody
      • GFR alpha1 antibody
      • GFR-alpha-1 antibody
      • GFRA 1 antibody
      • Gfra1 antibody
      • GFRA1_HUMAN antibody
      • GFRalpha1 antibody
      • Glial cell line derived neurotrophic factor receptor alpha antibody
      • GPI linked anchor protein antibody
      • MGC23045 antibody
      • PI linked cell surface accessory protein antibody
      • RET 1L antibody
      • RET ligand 1 antibody
      • RET1L antibody
      • RETL 1 antibody
      • RETL1 antibody
      • TGF beta related neurotrophic factor receptor 1 antibody
      • TGF-beta-related neurotrophic factor receptor 1 antibody
      • TRNR 1 antibody
      • TRNR1 antibody
      see all

    Images

    • All lanes : Anti-GDNF Receptor alpha 1 antibody (ab8026) at 1/500 dilution

      Lane 1 : Crude membrane fractions of human brain
      Lane 2 : Crude membrane fractions of liver
      Lane 3 : Crude membrane fractions of kidney
      Lane 4 : Crude membrane fractions of spleen


      Predicted band size : 50 kDa
      Observed band size : 50 kDa

    References

    This product has been referenced in:
    • Bellaïche J  et al. Gdnf-Gfra1 pathway is expressed in a spermatogenetic-dependent manner and is regulated by fsh in a fish testis. Biol Reprod 91:94 (2014). Read more (PubMed: 25165121) »
    • He Z  et al. Isolation, characterization, and culture of human spermatogonia. Biol Reprod 82:363-72 (2010). IHC-P ; Human . Read more (PubMed: 19846602) »

    See all 3 Publications for this product

    Customer reviews and Q&As

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Brain)
    Specification
    Brain
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    None
    Permeabilization
    Yes - 0.1% triton x-100
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    Submitted Oct 13 2008

    Thank you for your enquiry. I can confirm that the epitope of ab8026 GDNF Receptor alpha 1 antibody is predicted to be extracellular. We recommend a citrate antigen retrieval method for this antibody. I hope this is helpful. Should you have any ...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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