The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC (PFA fixed)
IHC-P: Use at a concentration of 1 µg/ml. Perform heat mediated antigen retrieval (citrate buffer 10 mM, pH 6.0) before commencing with IHC staining protocol.
Immunohistochemistry (PFA fixed): 1/50.
WB: 1/500. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Receptor for GDNF. Mediates the GDNF-induced autophosphorylation and activation of the RET receptor.