Overview

  • Product nameAnti-GEFT antibody
    See all GEFT primary antibodies
  • Description
    Rabbit polyclonal to GEFT
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Spider Monkey
  • Immunogen

    Synthetic peptide corresponding to a region within internal amino acids 504-553 (RIRLGDQAQGSTHTPINGSLPSLLLSPKGEVARALLPLDKQALGDIPQA P) of Human GEFT (NP_891992).

  • Positive control
    • Fetal brain lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab81684 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 64 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:312500.

Target

  • FunctionMay play a role in actin cytoskeleton reorganization in different tissues since its activation induces formation of actin stress fibers. It works as a guanine nucleotide exchange factor for Rho family of small GTPases. Links specifically G alpha q/11-coupled receptors to RHOA activation. May be an important regulator of processes involved in axon and dendrite formation. In neurons seems to be an exchange factor primarily for RAC1. Involved in skeletal myogenesis.
  • Tissue specificityIsoform 1 and isoform 2 are highly expressed in excitable tissues, such as brain, heart and muscle. Also detected in kidney and liver.
  • Sequence similaritiesContains 1 DH (DBL-homology) domain.
    Contains 1 PH domain.
  • DomainThe guanine nucleotide exchange activity is autoinhibited by the PH domain.
  • Cellular localizationCell membrane. Cytoplasm > myofibril > sarcomere. Highly colocalizes with actin regions.
  • Information by UniProt
  • Database links
  • Alternative names
    • ARHGEF25 antibody
    • ARHGP_HUMAN antibody
    • GEFT antibody
    • Guanine nucleotide exchange factor GEFT antibody
    • p63RhoGEF antibody
    • RAC/CDC42 exchange factor antibody
    • Rac/Cdc42/Rho exchange factor GEFT antibody
    • Rho guanine nucleotide exchange factor 25 antibody
    • RhoA/RAC/CDC42 exchange factor antibody
    • RhoA/Rac/Cdc42 guanine nucleotide exchange factor GEFT antibody
    see all

Anti-GEFT antibody images

  • Anti-GEFT antibody (ab81684) at 1 µg/ml (in 5% skim milk / PBS buffer) + fetal brain lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG should be diluted 1/50,000 - 1/100,000 as secondary antibody.

    Predicted band size : 64 kDa
    Observed band size : 64 kDa
    Additional bands at : 65 kDa,70 kDa. We are unsure as to the identity of these extra bands.

References for Anti-GEFT antibody (ab81684)

ab81684 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"