Overview

  • Product nameAnti-Gelsolin antibody
    See all Gelsolin primary antibodies
  • Description
    Rabbit polyclonal to Gelsolin
  • Tested applicationsSuitable for: WB, IHC-P, ICC, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant Human Gelsolin protein.

  • Positive control
    • Human Tonsil tissue; H1299 and Hela whole cell lysates; HeLa cells.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab113229 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 86 kDa.
IHC-P Use a concentration of 10 µg/ml.
ICC 1/100 - 1/200.
ICC/IF 1/100 - 1/200.

Target

  • FunctionCalcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis.
  • Tissue specificityPhagocytic cells, platelets, fibroblasts, nonmuscle cells, smooth and skeletal muscle cells.
  • Involvement in diseaseDefects in GSN are the cause of amyloidosis type 5 (AMYL5) [MIM:105120]; also known as familial amyloidosis Finnish type. AMYL5 is a hereditary generalized amyloidosis due to gelsolin amyloid deposition. It is typically characterized by cranial neuropathy and lattice corneal dystrophy. Most patients have modest involvement of internal organs, but severe systemic disease can develop in some individuals causing peripheral polyneuropathy, amyloid cardiomyopathy, and nephrotic syndrome leading to renal failure.
  • Sequence similaritiesBelongs to the villin/gelsolin family.
    Contains 6 gelsolin-like repeats.
  • Post-translational
    modifications
    Phosphorylation on Tyr-86, Tyr-409, Tyr-465, Tyr-603 and Tyr-651 in vitro is induced in presence of phospholipids.
  • Cellular localizationCytoplasm > cytoskeleton and Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Actin depolymerizing factor antibody
    • Actin-depolymerizing factor antibody
    • ADF antibody
    • AGEL antibody
    • Brevin antibody
    • DKFZp313L0718 antibody
    • GELS_HUMAN antibody
    • Gelsolin antibody
    • Gsn antibody
    see all

Anti-Gelsolin antibody images

  • ab113229, at 10µg/ml, staining Gelsolin in Formalin-fixed, Paraffin-embedded Human Tonsil tissue by Immunohistochemistry.
  • All lanes : Anti-Gelsolin antibody (ab113229) at 1/1000 dilution

    Lane 1 : H1299 whole cell lysate
    Lane 2 : HeLa whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 86 kDa
  • ab113229 at 1/200 dilution staining Gelsolin in methanol-fixed HeLa cells by Immunofluorescence. The lower image is co-stained with Hoeschst 33342.

References for Anti-Gelsolin antibody (ab113229)

ab113229 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"