Anti-Gelsolin antibody (ab53560)

Overview

• Product nameAnti-Gelsolin antibody
  See all Gelsolin primary antibodies
• Description
  Goat polyclonal to Gelsolin
• SpecificityThis antibody is expected to recognize both reported isoforms (NP_000168.1 and NP_937895.1).
• Tested applicationsSuitable for: WBmore details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse, Rat, Horse, Cow, Dog, Pig
• Immunogen

  Synthetic peptide:

  C-PRLKDKKMDAHP

  , corresponding to internal sequence amino acids 655-666 of Human Gelsolin
  Run BLAST with Run BLAST with
• Positive control
  • Human Spleen lysate.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 0.5% BSA, Tris saline, pH 7.3
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• ClonalityPolyclonal
• IsotypeIgG
• Research areas
  • Signal Transduction
  • Cytoskeleton / ECM
  • Cytoskeleton
  • Microfilaments
  • Actin etc
  • Actin Binding Proteins

Associated products

• Compatible Secondaries
  • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
  • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
• Positive Controls
  • Recombinant Human Gelsolin protein (ab114279)

Applications

Target

• FunctionCalcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis.
• Tissue specificityPhagocytic cells, platelets, fibroblasts, nonmuscle cells, smooth and skeletal muscle cells.
• Involvement in diseaseDefects in GSN are the cause of amyloidosis type 5 (AMYL5) [MIM:105120]; also known as familial amyloidosis Finnish type. AMYL5 is a hereditary generalized amyloidosis due to gelsolin amyloid deposition. It is typically characterized by cranial neuropathy and lattice corneal dystrophy. Most patients have modest involvement of internal organs, but severe systemic disease can develop in some individuals causing peripheral polyneuropathy, amyloid cardiomyopathy, and nephrotic syndrome leading to renal failure.
• Sequence similaritiesBelongs to the villin/gelsolin family.
  Contains 6 gelsolin-like repeats.
• Post-translational
  modificationsPhosphorylation on Tyr-86, Tyr-409, Tyr-465, Tyr-603 and Tyr-651 in vitro is induced in presence of phospholipids.
• Cellular localizationCytoplasm > cytoskeleton and Secreted.

• Target information above from: UniProt accession P06396 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• Database links
  • Entrez Gene: 535077 Cow
  • Entrez Gene: 100034186 Horse
  • Entrez Gene: 2934 Human
  • Entrez Gene: 227753 Mouse
  • Entrez Gene: 396874 Pig
  • Entrez Gene: 296654 Rat
  • Omim: 137350 Human
  • SwissProt: Q3SX14 Cow

  • SwissProt: Q28372 Horse
  • SwissProt: P06396 Human
  • SwissProt: P13020 Mouse
  • SwissProt: P20305 Pig
  • SwissProt: Q68FP1 Rat
  • Unigene: 522373 Human
  • Unigene: 21109 Mouse
  • Unigene: 103770 Rat

  see all
• Alternative names
  • Actin depolymerizing factor antibody
  • Actin-depolymerizing factor antibody
  • ADF antibody

  • AGEL antibody
  • Brevin antibody
  • DKFZp313L0718 antibody
  • GELS_HUMAN antibody
  • Gelsolin antibody
  • Gsn antibody

  see all