- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-Gelsolin antibody
See all Gelsolin primary antibodies -
DescriptionGoat polyclonal to Gelsolin
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Host speciesGoat
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SpecificityThis antibody is expected to recognize both reported isoforms (NP_000168.1 and NP_937895.1).
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Tested applicationsSuitable for: WBmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Horse, Cow, Dog, Pig
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Immunogen
Synthetic peptide corresponding to Human Gelsolin aa 655-666 (internal sequence).
Sequence:C-PRLKDKKMDAHP
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Positive control
- Human Spleen lysate.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris saline, pH 7.3 -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab53560 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 86 kDa). |
Target
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FunctionCalcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis.
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Tissue specificityPhagocytic cells, platelets, fibroblasts, nonmuscle cells, smooth and skeletal muscle cells.
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Involvement in diseaseDefects in GSN are the cause of amyloidosis type 5 (AMYL5) [MIM:105120]; also known as familial amyloidosis Finnish type. AMYL5 is a hereditary generalized amyloidosis due to gelsolin amyloid deposition. It is typically characterized by cranial neuropathy and lattice corneal dystrophy. Most patients have modest involvement of internal organs, but severe systemic disease can develop in some individuals causing peripheral polyneuropathy, amyloid cardiomyopathy, and nephrotic syndrome leading to renal failure.
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Sequence similaritiesBelongs to the villin/gelsolin family.
Contains 6 gelsolin-like repeats. -
Post-translational
modificationsPhosphorylation on Tyr-86, Tyr-409, Tyr-465, Tyr-603 and Tyr-651 in vitro is induced in presence of phospholipids. -
Cellular localizationCytoplasm > cytoskeleton and Secreted.
- Information by UniProt
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Database links
- Entrez Gene: 535077 Cow
- Entrez Gene: 100034186 Horse
- Entrez Gene: 2934 Human
- Entrez Gene: 227753 Mouse
- Entrez Gene: 396874 Pig
- Entrez Gene: 296654 Rat
- Omim: 137350 Human
- SwissProt: Q3SX14 Cow
see all -
Alternative names
- Actin depolymerizing factor antibody
- Actin-depolymerizing factor antibody
- ADF antibody
see all
Images
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Western blot - Anti-Gelsolin antibody (ab53560)Anti-Gelsolin antibody (ab53560) at 0.1 µg/ml + Human Spleen lysate (35µg protein in RIPA buffer).
Predicted band size: 86 kDa
Observed band size: 90 kDa (why is the actual band size different from the predicted?)
Primary incubation was 1 hour. Detected by chemiluminescence.
Protocols
References
ab53560 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"