Anti-Gelsolin antibody (ab53560)

Overview

• Product name
  Anti-Gelsolin antibody
  See all Gelsolin primary antibodies
• Description
  Goat polyclonal to Gelsolin
• Specificity
  This antibody is expected to recognize both reported isoforms (NP_000168.1 and NP_937895.1).
• Tested applications
  Suitable for: WBmore details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse, Rat, Horse, Cow, Dog, Pig
• Immunogen

  Synthetic peptide:

  C-PRLKDKKMDAHP

  , corresponding to internal sequence amino acids 655-666 of Human Gelsolin

  Run BLAST with Run BLAST with
• Positive control
  • Human Spleen lysate.

Properties

• Form
  Liquid
• Storage instructions
  Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage buffer
  Preservative: 0.02% Sodium Azide
  Constituents: 0.5% BSA, Tris saline, pH 7.3
• Concentration information loading...
• Purity
  Immunogen affinity purified
• Purification notes
  Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Clonality
  Polyclonal
• Isotype
  IgG
• Research areas
  • Signal Transduction
  • Cytoskeleton / ECM
  • Cytoskeleton
  • Microfilaments
  • Actin etc
  • Actin Binding Proteins

Associated products

• Compatible Secondaries
  • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
  • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
• Positive Controls
  • Recombinant Human Gelsolin protein (ab114279)

Applications

Target

• Function
  Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis.
• Tissue specificity
  Phagocytic cells, platelets, fibroblasts, nonmuscle cells, smooth and skeletal muscle cells.
• Involvement in disease
  Defects in GSN are the cause of amyloidosis type 5 (AMYL5) [MIM:105120]; also known as familial amyloidosis Finnish type. AMYL5 is a hereditary generalized amyloidosis due to gelsolin amyloid deposition. It is typically characterized by cranial neuropathy and lattice corneal dystrophy. Most patients have modest involvement of internal organs, but severe systemic disease can develop in some individuals causing peripheral polyneuropathy, amyloid cardiomyopathy, and nephrotic syndrome leading to renal failure.
• Sequence similarities
  Belongs to the villin/gelsolin family.
  Contains 6 gelsolin-like repeats.
• Post-translational
  modifications
  Phosphorylation on Tyr-86, Tyr-409, Tyr-465, Tyr-603 and Tyr-651 in vitro is induced in presence of phospholipids.
• Cellular localization
  Cytoplasm > cytoskeleton and Secreted.

• Target information above from: UniProt accession P06396 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• Database links
  • Entrez Gene: 535077 Cow
  • Entrez Gene: 100034186 Horse
  • Entrez Gene: 2934 Human
  • Entrez Gene: 227753 Mouse
  • Entrez Gene: 396874 Pig
  • Entrez Gene: 296654 Rat
  • Omim: 137350 Human
  • SwissProt: Q3SX14 Cow

  • SwissProt: Q28372 Horse
  • SwissProt: P06396 Human
  • SwissProt: P13020 Mouse
  • SwissProt: P20305 Pig
  • SwissProt: Q68FP1 Rat
  • Unigene: 522373 Human
  • Unigene: 21109 Mouse
  • Unigene: 103770 Rat

  see all
• Alternative names
  • Actin depolymerizing factor antibody
  • Actin-depolymerizing factor antibody
  • ADF antibody

  • AGEL antibody
  • Brevin antibody
  • DKFZp313L0718 antibody
  • GELS_HUMAN antibody
  • Gelsolin antibody
  • Gsn antibody

  see all

Protocols

• Western blot protocols