The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 86 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use a concentration of 5 µg/ml.
FunctionCalcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis.
Involvement in diseaseDefects in GSN are the cause of amyloidosis type 5 (AMYL5) [MIM:105120]; also known as familial amyloidosis Finnish type. AMYL5 is a hereditary generalized amyloidosis due to gelsolin amyloid deposition. It is typically characterized by cranial neuropathy and lattice corneal dystrophy. Most patients have modest involvement of internal organs, but severe systemic disease can develop in some individuals causing peripheral polyneuropathy, amyloid cardiomyopathy, and nephrotic syndrome leading to renal failure.
Sequence similaritiesBelongs to the villin/gelsolin family. Contains 6 gelsolin-like repeats.
Post-translational modificationsPhosphorylation on Tyr-86, Tyr-409, Tyr-465, Tyr-603 and Tyr-651 in vitro is induced in presence of phospholipids.
Cellular localizationCytoplasm > cytoskeleton and Secreted.
ab76418 at 5µg/ml staining Gelsolin in tonsil tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Western blot - Gelsolin antibody (ab76418)
Anti-Gelsolin antibody (ab76418) at 1 µg/ml + MCF7 cell lysate at 10 µg
Secondary HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 86 kDa Observed band size : 86 kDa
Western blot - Anti-Gelsolin antibody (ab76418)
Predicted band size : 86 kDa Commercial M1 and M2 are cell culture supernatants of human monocyte derived macrophages.
References for Anti-Gelsolin antibody (ab76418)
This product has been referenced in:
Bewley MA et al. Proteomic evaluation and validation of cathepsin D regulated proteins in macrophages exposed to Streptococcus pneumoniae. Mol Cell Proteomics10:M111.008193 (2011).
Read more (PubMed: 21474794) »
Haverland N et al. Immunoreactivity of anti-gelsolin antibodies: implications for biomarker validation. J Transl Med8:137 (2010).
Read more (PubMed: 21171974) »