• Product nameAnti-Gelsolin antibody [EPR1941Y]
    See all Gelsolin primary antibodies
  • Description
    Rabbit monoclonal [EPR1941Y] to Gelsolin
  • SpecificityThe antibody only detects the isoform 1 of the protein (86kDa).
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues in the N terminal region of human Gelsolin.

  • Positive control
    • Human plasma
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.


Associated products


Our Abpromise guarantee covers the use of ab75832 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/25000 - 1/50000. Detects a band of approximately 90 kDa (predicted molecular weight: 86 kDa).
IP 1/50.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
  • Application notesIs unsuitable for Flow Cyt or ICC.
  • Target

    • FunctionCalcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis.
    • Tissue specificityPhagocytic cells, platelets, fibroblasts, nonmuscle cells, smooth and skeletal muscle cells.
    • Involvement in diseaseDefects in GSN are the cause of amyloidosis type 5 (AMYL5) [MIM:105120]; also known as familial amyloidosis Finnish type. AMYL5 is a hereditary generalized amyloidosis due to gelsolin amyloid deposition. It is typically characterized by cranial neuropathy and lattice corneal dystrophy. Most patients have modest involvement of internal organs, but severe systemic disease can develop in some individuals causing peripheral polyneuropathy, amyloid cardiomyopathy, and nephrotic syndrome leading to renal failure.
    • Sequence similaritiesBelongs to the villin/gelsolin family.
      Contains 6 gelsolin-like repeats.
    • Post-translational
      Phosphorylation on Tyr-86, Tyr-409, Tyr-465, Tyr-603 and Tyr-651 in vitro is induced in presence of phospholipids.
    • Cellular localizationCytoplasm > cytoskeleton and Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • Actin depolymerizing factor antibody
      • Actin-depolymerizing factor antibody
      • ADF antibody
      • AGEL antibody
      • Brevin antibody
      • DKFZp313L0718 antibody
      • GELS_HUMAN antibody
      • Gelsolin antibody
      • Gsn antibody
      see all

    Anti-Gelsolin antibody [EPR1941Y] images

    • Lanes 1 - 4 : Anti-Gelsolin antibody [EPR1941Y] (ab75832) at 1/10000 dilution
      Lanes 5 - 8 : Anti-Gelsolin antibody [EPR1942] (ab109014) at 1/20000 dilution
      Lanes 9 - 12 : Anti-Gelsolin antibody [EP1940Y] (ab134183) at 1/10000 dilution

      Lane 1 : THP-1 (human acute monocytic leukemia) whole cell lysate
      Lane 2 : THP-1 cultured medium
      Lane 3 : MCF-7 (human breast adenocarcinoma) whole cell lysate
      Lane 4 : Human Plasma
      Lane 5 : THP-1 (human acute monocytic leukemia) whole cell lysate
      Lane 6 : THP-1 cultured medium
      Lane 7 : MCF-7 (human breast adenocarcinoma) whole cell lysate
      Lane 8 : Human Plasma
      Lane 9 : THP-1 (human acute monocytic leukemia) whole cell lysate
      Lane 10 : THP-1 cultured medium
      Lane 11 : MCF-7 (human breast adenocarcinoma) whole cell lysate
      Lane 12 : Human Plasma

      Lysates/proteins at 10 µg per lane.

      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 86 kDa
      Observed band size : 81/86 kDa (why is the actual band size different from the predicted?)

      Exposure time : 10 seconds

      Bloking buffer 5% NFDM/TBST
      Diluting buffer 5% NFDM/TBST

      The secretory isoform of gelsolin is at 86kDa. The cytoplasmic isoforms of gelsolin are at 81kDa. Lane 5-8: ab109014 detected both secretory and cytoplasmic isoforms. Lane 9-12: ab134183 detected both secretory and cytoplasmic isoforms.

    • Anti-Gelsolin antibody [EPR1941Y] (ab75832) at 1/50000 dilution + human plasma at 10 µg

      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 86 kDa
      Observed band size : 90 kDa (why is the actual band size different from the predicted?)
    • ab75832 Immunoprecipitating Gelsolin in mouse embryo whole cell lysate. 2µg of capture antibody (ab75832) per 0.35mg of lysate was used. 10µg of plasma was incubated with primary antibody (Panel A: ab75832, Panel B: ab134183) at a dilution of 1/1000 and VeriBlot for IP secondary antibody (HRP) ab131366 was used as the secondary antibody at a dilution of 1/10000.

      Lane 1: Human plasma
      Lane 2: Human plasma
      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab75832 in Human plasma.

      Exposure time: Panel A - 8 seconds, Panel B - 3 minutes.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      The additional band at 50 kDa is a rabbit IgG heavy chain.

    • ab75832 at 1/100 dilution staining Gelsolin in human colon tissue, using a HRP/AP polymerized secondary antibody.

    References for Anti-Gelsolin antibody [EPR1941Y] (ab75832)

    This product has been referenced in:
    • Griffoni C  et al. Modification of proteins secreted by endothelial cells during modeled low gravity exposure. J Cell Biochem 112:265-72 (2011). WB ; Human . Read more (PubMed: 21069737) »
    • Welander H  et al. Gelsolin co-occurs with Lewy bodies in vivo and accelerates a-synuclein aggregation in vitro. Biochem Biophys Res Commun 412:32-8 (2011). ICC/IF ; Human . Read more (PubMed: 21798243) »

    See all 2 Publications for this product

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