Recombinant
RabMAb

Anti-Gelsolin plasma antibody [EPR1941Y] (ab75832)

Overview

  • Product name
    Anti-Gelsolin plasma antibody [EPR1941Y]
    See all Gelsolin plasma primary antibodies
  • Description
    Rabbit monoclonal [EPR1941Y] to Gelsolin plasma
  • Host species
    Rabbit
  • Specificity
    The antibody only detects the isoform 1 of the protein (86kDa).
  • Tested applications
    Suitable for: WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Gelsolin plasma aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: P06396-1

  • Positive control
    • Human plasma
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab75832 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/25000 - 1/50000. Detects a band of approximately 90 kDa (predicted molecular weight: 86 kDa).
IP 1/50.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt or ICC.
  • Target

    • Relevance
      Gelsolin is a calcium regulated, actin modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. It plays a role in phosphoinositide mediated disassembly of actin filaments in Sertoli cell adhesion complexes; it may regulate sperm release and turnover of the blood testis barrier [RGD], and severs actin filaments in the presence of submicromolar calcium, thereby solating cytoplasmic actin gels. A calcium independent mechanism reverses the process. Northern blot analysis suggested that a single gene encodes both cell and plasma gelsolins. This protein may be unique in that it is made for both secretion and intracytoplasmic location.
    • Cellular localization
      Secreted
    • Database links
    • Alternative names
      • Actin depolymerizing factor antibody
      • ADF antibody
      • AGEL antibody
      • Brevin antibody
      • Gelsolin antibody
      see all

    Images

    • Lanes 1-4 : Anti-Gelsolin plasma antibody [EPR1941Y] (ab75832) at 1/10000 dilution
      Lanes 5-8 : Anti-Gelsolin antibody [EPR1942] (ab109014) at 1/20000 dilution
      Lanes 9-12 : Anti-Gelsolin antibody [EP1940Y] (ab134183) at 1/10000 dilution

      Lanes 1 & 5 & 9 : THP-1 (human acute monocytic leukemia) whole cell lysate
      Lanes 2 & 6 & 10 : THP-1 cultured medium
      Lanes 3 & 7 & 11 : MCF-7 (human breast adenocarcinoma) whole cell lysate
      Lanes 4 & 8 & 12 : Human Plasma

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 86 kDa
      Observed band size: 81/86 kDa (why is the actual band size different from the predicted?)


      Exposure time: 10 seconds


      Bloking buffer 5% NFDM/TBST
      Diluting buffer 5% NFDM/TBST

      The secretory isoform of gelsolin is at 86kDa. The cytoplasmic isoforms of gelsolin are at 81kDa. Lane 5-8: ab109014 detected both secretory and cytoplasmic isoforms. Lane 9-12: ab134183 detected both secretory and cytoplasmic isoforms.

    • Anti-Gelsolin plasma antibody [EPR1941Y] (ab75832) at 1/50000 dilution + human plasma at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 86 kDa
      Observed band size: 90 kDa (why is the actual band size different from the predicted?)

    • ab75832 Immunoprecipitating Gelsolin plasma in mouse embryo whole cell lysate. 2µg of capture antibody (ab75832) per 0.35mg of lysate was used. 10µg of plasma was incubated with primary antibody (Panel A: ab75832, Panel B: ab134183) at a dilution of 1/1000 and VeriBlot for IP secondary antibody (HRP) ab131366 was used as the secondary antibody at a dilution of 1/10000.

      Lane 1: Human plasma
      Lane 2: Human plasma
      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab75832 in Human plasma.

      Exposure time: Panel A - 8 seconds, Panel B - 3 minutes.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      The additional band at 50 kDa is a rabbit IgG heavy chain.

    • ab75832 at 1/100 dilution staining Gelsolin plasma in human colon tissue, using a HRP/AP polymerized secondary antibody.

    References

    This product has been referenced in:
    • Hashimoto K  et al. The potential of neurotensin secreted from neuroendocrine tumor cells to promote gelsolin-mediated invasiveness of prostate adenocarcinoma cells. Lab Invest 95:283-95 (2015). Read more (PubMed: 25581609) »
    • Polioudakis D  et al. MiR-191 Regulates Primary Human Fibroblast Proliferation and Directly Targets Multiple Oncogenes. PLoS One 10:e0126535 (2015). WB . Read more (PubMed: 25992613) »

    See all 4 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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