The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 34 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
GEM belongs to the RAD/GEM family of GTP-binding proteins. It could be a regulatory protein, possibly participating in receptor-mediated signal transduction at the plasma membrane. GEM has guanine nucleotide-binding activity but undetectable intrinsic GTPase activity. Alternative splicing occurs at this locus and two transcript variants encoding the same protein have been identified.