Primary antibody notesInvolved in biogenesis of spliceosomal snRNPs, forms complex with Survival of Motor Neurons (SMN) protein, interacts tightly with spliceosomal proteins and spliceosomal snRNAs U1 and U5.
Overlay histogram showing HeLa cells stained with ab6084 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab6084, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Immunocytochemistry/ Immunofluorescence - Anti-Gemin 2 antibody [2E17] (ab6084)Image from Morency E et al, J Cell Biol. 2007 Jun 4;177(5):757-68, Fig 4f. DOI 10.1083/jcb.200612107.
HeLa cells were transfected with an ICP0-expressing plasmid before IF assays to detect the distribution of CB- and gem-associated proteins. ab6084 used at a 1/200 dilution to detect Gemin 2 (green). Bar 5 µm.
References for Anti-Gemin 2 antibody [2E17] (ab6084)
This product has been referenced in:
Groen EJ et al. ALS-associated mutations in FUS disrupt the axonal distribution and function of SMN. Hum Mol GenetN/A:N/A (2013).
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