Overview

  • Product name
    Anti-Gemin 2 antibody [2E17]
    See all Gemin 2 primary antibodies
  • Description
    Mouse monoclonal [2E17] to Gemin 2
  • Tested applications
    Suitable for: Flow Cyt, ICC/IF, ELISA, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human, Xenopus laevis
  • Immunogen

    His-Gemin 2 fusion protein (amino acids 12 - 280, starting at 2nd methionine).

  • General notes


    Involved in biogenesis of spliceosomal snRNPs, forms complex with Survival of Motor Neurons (SMN) protein, interacts tightly with spliceosomal proteins and spliceosomal snRNAs U1 and U5.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Purification notes
    Purified from supernatant.
  • Primary antibody notes
    Involved in biogenesis of spliceosomal snRNPs, forms complex with Survival of Motor Neurons (SMN) protein, interacts tightly with spliceosomal proteins and spliceosomal snRNAs U1 and U5.
  • Clonality
    Monoclonal
  • Clone number
    2E17
  • Myeloma
    Sp2/0
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6084 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells.
ICC/IF Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 34 kDa.
IP Use at an assay dependent concentration.

Target

  • Function
    The SMN complex plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing in the nucleus.
  • Sequence similarities
    Belongs to the gemin-2 family.
  • Cellular localization
    Nucleus > gem. Cytoplasm. Localized in subnuclear structures next to coiled bodies, called gems, which are highly enriched in spliceosomal snRNPs. Also found in the cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Component of gems 2 antibody
    • Gem (nuclear organelle) associated protein 2 antibody
    • Gem associated protein 2 antibody
    • GEMI2_HUMAN antibody
    • Gemin-2 antibody
    • gemin2 antibody
    • SIP 1 antibody
    • SIP-1 antibody
    • SIP1 antibody
    • SIP1 delta antibody
    • SIP1-delta antibody
    • SMN interacting protein 1 antibody
    • SMN interacting protein 1 delta antibody
    • SMN-interacting protein 1 antibody
    • Survival interacting protein 1 antibody
    • Survival of motor neuron protein interacting protein 1 antibody
    • Survival of motor neuron protein-interacting protein 1 antibody
    see all

Images

  • Overlay histogram showing HeLa cells stained with ab6084 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab6084, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • HeLa cells were transfected with an ICP0-expressing plasmid before IF assays to detect the distribution of CB- and gem-associated proteins. ab6084 used at a 1/200 dilution to detect Gemin 2 (green). Bar 5 µm.

References

This product has been referenced in:
  • Staropoli JF  et al. Rescue of gene-expression changes in an induced mouse model of spinal muscular atrophy by an antisense oligonucleotide that promotes inclusion of SMN2 exon 7. Genomics 105:220-8 (2015). Read more (PubMed: 25645699) »
  • Groen EJ  et al. ALS-associated mutations in FUS disrupt the axonal distribution and function of SMN. Hum Mol Genet N/A:N/A (2013). Read more (PubMed: 23681068) »

See all 8 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Hela cells)
Specification
Hela cells
Fixative
Methanol
Permeabilization
No
Blocking step
Fish scale gelatin in PBS as blocking agent for 20 minute(s) · Concentration: 0.2% · Temperature: 25°C
Username

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Verified customer

Submitted Jul 24 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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