Overview

  • Product nameAnti-Gemin 2 antibody [2E17]
    See all Gemin 2 primary antibodies
  • Description
    Mouse monoclonal [2E17] to Gemin 2
  • Tested applicationsSuitable for: Flow Cyt, ICC/IF, ELISA, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human, Xenopus laevis
  • Immunogen

    His-Gemin 2 fusion protein (amino acids 12 - 280, starting at 2nd methionine).

  • General notes


    Involved in biogenesis of spliceosomal snRNPs, forms complex with Survival of Motor Neurons (SMN) protein, interacts tightly with spliceosomal proteins and spliceosomal snRNAs U1 and U5.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein A purified
  • Purification notesPurified from supernatant.
  • Primary antibody notesInvolved in biogenesis of spliceosomal snRNPs, forms complex with Survival of Motor Neurons (SMN) protein, interacts tightly with spliceosomal proteins and spliceosomal snRNAs U1 and U5.
  • ClonalityMonoclonal
  • Clone number2E17
  • MyelomaSp2/0
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6084 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells.
ICC/IF Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 34 kDa.
IP Use at an assay dependent concentration.

Target

  • FunctionThe SMN complex plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing in the nucleus.
  • Sequence similaritiesBelongs to the gemin-2 family.
  • Cellular localizationNucleus > gem. Cytoplasm. Localized in subnuclear structures next to coiled bodies, called gems, which are highly enriched in spliceosomal snRNPs. Also found in the cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Component of gems 2 antibody
    • Gem (nuclear organelle) associated protein 2 antibody
    • Gem associated protein 2 antibody
    • GEMI2_HUMAN antibody
    • Gemin-2 antibody
    • gemin2 antibody
    • SIP 1 antibody
    • SIP-1 antibody
    • SIP1 antibody
    • SIP1 delta antibody
    • SIP1-delta antibody
    • SMN interacting protein 1 antibody
    • SMN interacting protein 1 delta antibody
    • SMN-interacting protein 1 antibody
    • Survival interacting protein 1 antibody
    • Survival of motor neuron protein interacting protein 1 antibody
    • Survival of motor neuron protein-interacting protein 1 antibody
    see all

Anti-Gemin 2 antibody [2E17] images

  • Overlay histogram showing HeLa cells stained with ab6084 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab6084, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • HeLa cells were transfected with an ICP0-expressing plasmid before IF assays to detect the distribution of CB- and gem-associated proteins. ab6084 used at a 1/200 dilution to detect Gemin 2 (green). Bar 5 µm.

References for Anti-Gemin 2 antibody [2E17] (ab6084)

This product has been referenced in:

See all 7 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Hela cells)
Specification Hela cells
Fixative Methanol
Permeabilization No
Blocking step Fish scale gelatin in PBS as blocking agent for 20 minute(s) · Concentration: 0.2% · Temperature: 25°C
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Submitted Jul 24 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"